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目的:研究芩百清肺浓缩丸高效液相色谱指纹图谱,建立整体特征的质量评价方法。方法:采用UV-ELSD串联检测技术,Zorbax色谱柱,以乙腈-1.5%冰醋酸为流动相,梯度洗脱,检测波长280nm。结果:本色谱条件下芩百清肺浓缩丸各成分得到了较好的分离,标准指纹图谱均由10个特征峰组成,相似度>0.05。结论:所建立的HPLC指纹图谱表达了芩百清肺浓缩丸可测组分的整体特征。具有重复性好、特征性强、方法简便等特点,可用于芩百清肺浓缩丸的质量评价。
Objective: To study the HPLC fingerprint of Qinbaiqing Pneumoconiosis Pills and to establish the quality evaluation method of the whole features. Methods: UV-ELSD tandem detection was performed on a Zorbax column with acetonitrile-1.5% glacial acetic acid as mobile phase and gradient elution with a detection wavelength of 280 nm. Results: The components of Qinbaiqing Pneumoconiosis Pill under the chromatographic conditions were well separated. The standard fingerprints consisted of 10 characteristic peaks with similarity> 0.05. Conclusion: The established HPLC fingerprints express the general characteristics of the measurable component of Qinbai Qingfei Pills. It has the characteristics of good repeatability, strong characteristic and simple method, which can be used for the quality evaluation of Qinbaiqing Pulmonary Concentrated Pills.