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目的 :探讨非霍奇金氏淋巴瘤 (NHL)病理特点、免疫表型及与慢性淋巴腺炎和增生性淋巴腺炎 (RH)免疫表型的不同表达。方法 :对 2 0 6例淋巴结肿大患者进行淋巴结穿刺 ,取穿刺液进行染色镜检 ,作出初步诊断。而后采用流式细胞仪(FCM)检测各患者淋巴结穿刺液中淋巴细胞免疫表型。结果 :①经形态学检查慢性淋巴腺炎患者 95例 ;增生性淋巴腺炎患者4 0例 ;NHL患者 71例。② 71例NHL中B NHL 5 3例 ,占 74 6 4 % ;T NHL 12例 ,占 16 90 % ;CD3阳性 15例 ,占 2 1 13% ;CD4阳性6例 ,占 8 4 5 % ;CD34阳性 30例 ,占 4 2 2 5 %。③NHL各抗原表达结果与增生性淋巴腺炎、慢性淋巴腺炎各抗原表达结果均有显著差异 (P <0 0 5 )。结论 :免疫表型检查有助于确定绝大多数NHL病例、肿瘤增生细胞的T或B性质及克隆性 ,免疫表型检查可作为NHL鉴别诊断的辅助指标。
Objective: To investigate the pathological features, immunophenotypes and immunophenotypes of non-Hodgkin’s lymphoma (NHL) and their immunophenotypes with chronic lymphadenitis and proliferative lymphadenitis (RH). Methods: Twenty - six patients with lymphadenopathy underwent lymph node puncture, and puncture fluid was used for microscopic examination to make a preliminary diagnosis. Flow cytometry (FCM) was used to detect lymphocyte immunophenotype in lymph node aspiration fluid. Results: ① There were 95 cases of chronic lymphadenitis by morphological examination, 40 cases of proliferative lymphadenitis and 71 cases of NHL. ② Among 71 NHLs, 3 cases were NHL 5, accounting for 74.64%; 12 cases were T NHL, accounting for 16.9%; 15 cases were CD3 positive, accounting for 21.13%; 6 cases were CD4 positive, accounting for 84.5%; CD34 Positive in 30 cases, 4 2 2 5%. (3) The expression of each antigen of NHL was significantly different from that of proliferative lymphadenitis and chronic lymphocyticitis (P <0.05). Conclusion: Immunophenotyping can help determine the nature and clonality of T or B in most NHL cases and tumor cells. Immunophenotyping can be used as an adjunct to differential diagnosis of NHL.