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目的:探讨鼻咽癌(NPC)组织中胰岛素样生长因子1受体(IGF-1R)蛋白的表达及其临床意义。方法:应用免疫组织化学方法检测63例NPC组织和鼻咽部炎性黏膜组织中IGF-1R的表达,对其中45例NPC患者进行5年随访观察,以及采用免疫印迹法检测3种体外培养的NPC细胞系中IGF-1R蛋白的表达情况。结果:63例NPC和21例鼻咽黏膜(伴慢性炎症)组织中的IGF-1R阳性表达率分别为82.5%和14.3%,在NPC中的表达阳性率明显高于鼻咽炎性组织(P<0.01)。NPC组织中IGF-IR的表达与TNM分期和颈淋巴结转移有关,有颈淋巴结转移者其表达阳性率较高(P<0.05);Ⅰ~Ⅱ期表达阳性率较Ⅲ~Ⅳ期低(P<0.01);NPC局部复发者IGF-lR阳性表达率(89.5%)明显高于无局部复发者(53.9%),P<0.05;NPC放疗后生存期小于5年者的阳性表达率(88.9%)明显高于5年以上生存期者的阳性表达率(51.9%),P<0.01;病理分化程度越高IGF-lR阳性表达率越低,但NPC病理类型间比较差异无统计学意义(P>0.05)。在3种NPC细胞系中IGF-1R的表达明显高于鼻咽炎症黏膜组织,其中TW03表达最强,CNE2其次,CNEl表达稍弱。结论:NPC组织中存在IGF-1R的过表达现象,IGF-1R的异常表达与NPC的发生、发展存在一定的关系。IGF-1R可能是复发或转移性NPC治疗中一个潜在的靶分子。
Objective: To investigate the expression of insulin-like growth factor 1 receptor (IGF-1R) in nasopharyngeal carcinoma (NPC) and its clinical significance. Methods: Immunohistochemical method was used to detect the expression of IGF-1R in 63 cases of NPC tissues and nasopharyngeal mucosa. Fifty-five cases of NPC were followed up for 5 years. Western blotting was used to detect the expression of IGF- NPC cell line IGF-1R protein expression. Results: The positive rates of IGF-1R in 63 NPC and 21 nasopharyngeal mucosa with chronic inflammation were 82.5% and 14.3%, respectively, which were significantly higher in NPC than those in nasopharyngitis (P < 0.01). The expression of IGF-IR in NPC was correlated with TNM staging and cervical lymph node metastasis, and the positive rate was higher in cervical lymph node metastasis (P <0.05). The positive expression rate of stage Ⅰ-Ⅱ was lower than that in stage Ⅲ-Ⅳ (P < 0.01). The positive expression rate of IGF-IR in local recurrence of NPC was 89.5%, which was significantly higher than that of no local recurrence (53.9%, P <0.05). The positive expression rate of IGF-IR in NPC with recurrence less than 5 years after radiotherapy was 88.9% (51.9%) was significantly higher than that of patients with more than 5 years survival (P <0.01). The higher the pathological differentiation was, the lower the positive rate of IGF-IR was, but there was no significant difference between NPC pathological types (P> 0.05). The expression of IGF-1R in three kinds of NPC cell lines was significantly higher than that in nasopharyngeal inflammatory mucosa tissues, of which TW03 was the strongest, followed by CNE2 and CNE1 slightly weaker. CONCLUSIONS: The overexpression of IGF-1R exists in NPC tissues. The abnormal expression of IGF-1R is associated with the occurrence and development of NPC. IGF-1R may be a potential target in the treatment of recurrent or metastatic NPC.