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目的观察甲基苯丙胺(MA)与HIV-Tat蛋白协同作用致大鼠相关脑区活性氧(ROS)、谷胱甘肽过氧化物酶(GSH-PX)和超氧化物歧化酶(SOD)的变化,探讨协同作用对神经系统的影响。方法 50只健康雄性SD大鼠随机分为实验组:MA组(10mg/kg MA,每日两次腹腔注射,连续4d)、HIV-Tat组(10μg HIV-Tat注入大鼠脑纹状体)和MA+HIV-Tat组(按MA组注射MA 4d后按HIV-Tat组注入HIV-Tat);对照组:生理盐水腹腔注射或纹状体内注射。各组大鼠分别于注射结束后48h和7d处死,取各脑区脑组织制作匀浆;荧光分光光度计检测ROS含量,酶标仪检测GSH-PX和SOD吸光度,再根据蛋白质的浓度分别计算其活力。结果各实验组与对照组比较,各脑区ROS含量有不同程度增高,GSH-PX和SOD活力则有不同程度下降,差异具有统计学意义(P<0.05);其中MA+HIV-Tat组与MA组和HIV-Tat组比较,ROS含量升高显著,GSH-PX和SOD活力下降明显(P<0.01)。结论 MA和HIV-Tat协同作用能够产生大量的ROS,并降低GSH-PX和SOD的活力,揭示ROS、GSH-PX和SOD参与了MA与HIV-Tat的协同神经毒性作用。
Objective To observe the effects of methamphetamine (MA) and HIV-Tat protein on the activity of reactive oxygen species (ROS), glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) Changes, to explore the synergistic effect on the nervous system. Methods Fifty healthy male Sprague-Dawley rats were randomly divided into experimental group: MA group (10mg / kg MA twice daily for 4 days), HIV-Tat group (10μg HIV-Tat injected into rat brain striatum) And MA + HIV-Tat group (injected with HIV-Tat by HIV-Tat group after MA was injected for 4 days in MA group); control group: normal saline was injected intraperitoneally or injected into the striatum. The rats in each group were sacrificed 48h and 7d after the injection, and the brain tissue of each brain was homogenized. The content of ROS was detected by fluorescence spectrophotometer. The absorbance of GSH-PX and SOD were detected by microplate reader. Its vitality. Results Compared with the control group, ROS levels in various brain regions increased to some extent and the activities of GSH-PX and SOD decreased to some extent (P <0.05) In MA group and HIV-Tat group, the content of ROS increased significantly and the activities of GSH-PX and SOD decreased significantly (P <0.01). Conclusions The synergistic effect of MA and HIV-Tat can produce a large amount of ROS and decrease the activity of GSH-PX and SOD, indicating that ROS, GSH-PX and SOD are involved in the synergistic neurotoxic effect of MA and HIV-Tat.