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目的:观察银杏叶复方对急性全脑缺血再灌注大鼠下丘脑神经生长因子受体(NGFR)表达的影响。方法:21只SD大鼠随机分为3组,假手术组(Sham组)、缺血再灌注损伤组(I/R组)和治疗组(GBE组)。采用pulsinelli四血管法制作大鼠急性全脑缺血再灌注损伤动物模型。GBE组大鼠在造模前1个月至造模后1周灌服银杏叶复方。免疫组化染色脑组织切片。电镜观察下丘脑视上核与室旁核的NGFR阳性细胞,并在200μm×200μm的方格测微尺内计数形态清楚的NGFR阳性细胞数,计算细胞密度值。结果:GBE组大鼠下丘脑视上核、室旁核NGFR阳性细胞数比I/R组与Sham组明显增多(P<0.01)。结论:银杏叶复方可促进脑缺血再灌注损伤大鼠下丘脑NGFR的表达而产生神经保护作用。
Objective: To observe the effect of Ginkgo biloba Compound on the expression of nerve growth factor receptor (NGFR) in hypothalamus of rats with acute global cerebral ischemia and reperfusion. Methods: Twenty-one Sprague-Dawley rats were randomly divided into three groups: Sham group, I / R group and GBE group. A rat model of acute global cerebral ischemia-reperfusion injury was established by pulsinelli four-vessel method. Rats in GBE group were given gingko leaf compound for 1 month before modeling and 1 week after modeling. Immunohistochemical staining of brain tissue sections. The NGFR positive cells in the supraoptic nucleus and paraventricular nucleus of the hypothalamus were observed by electron microscope. The numbers of positive NGFR positive cells were counted in a 200 μm × 200 μm square micrometer to calculate the cell density. Results: The number of NGFR positive cells in hypothalamic supraoptic nucleus and paraventricular nucleus in GBE group was significantly higher than that in I / R group and Sham group (P <0.01). Conclusion: Ginkgo biloba compound can promote the neuroprotective effect of NGFR in the hypothalamus of rats with cerebral ischemia-reperfusion injury.