唾液酸免离心快速测定法的建立及初步临床应用

• 作 者: 李军 魏曙亚 候蓉 李树业 原荣 安小惠 何冰
• 机 构: 陕西省肿瘤防治研究所基础研究室!710061,陕西省肿瘤防治研究所基础研究室!710061,陕西省肿瘤防治研究所基础研究室!710061,陕西省肿瘤防治研究所基础研究室!710061,陕西省肿瘤防治研
• 出 处: 现代肿瘤医学
• 发表日期: 1997年04期
• 关键词: 唾液酸 快速测定法 临床应用 肿瘤检测 线性范围 线性关系 基层单位 蛋白沉淀 无显著性差异 有显著性差异

本文报告建立唾液酸免离心快速测定法,用本法检测了137例正常人和308例恶性肿瘤及其它疾病患者血清唾液酸的含量,结果表明,其对癌症检测的灵敏度为83.4%,高于原测定方法(P<0.05),特异性95.5%,与现有方法相比无显著性差异(P>0.05)。本法操作简便、快速、省时、经济,而且具有血清用量少,线性关系好,线性范围宽,重复性好等特点,可在临床及基层单位推广使用。

This paper reports the establishment of a rapid method for the determination of sialic acid by centrifugation. The sialic acid levels in serum of 137 normal people and 308 patients with malignant tumors and other diseases were measured by this method. The results showed that the sensitivity to cancer detection was 83.4%. The original determination method (P<0.05) had a specificity of 95.5%, and there was no significant difference compared with the existing method (P>0.05). This method is simple, rapid, time-saving, economical, and has the characteristics of less serum, good linearity, wide linear range, and good repeatability. It can be used in clinical and grassroots units.