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目的 :探讨人参水提液 (简称人参液 )对大鼠慢性绿脓杆菌 (PA)性肺炎模型的影响。方法 :采用 4种不同剂量的人参液皮下注射 ,观察用药两周后的疗效。结果 :4种剂量的人参液均可显著减少肺部大体观病变和肺脓肿率 (P <0 0 5) ;镜下观其病变程度与人参剂量呈负相关 (r=-0 95,P <0 0 1 ) ;人参Ⅰ (5mg/kg)、Ⅱ (2 5mg/kg)、Ⅳ (0 6 2 5mg/kg)组的肥大细胞数低于对照组 (P <0 0 5) ;Ⅱ、Ⅲ (1 2 5mg/kg)组的肺部细菌清除作用强于对照组 (P <0 0 5) ;Ⅱ、Ⅲ、Ⅳ组的PA特异性抗体应答发生下调 (P <0 0 1 ) ;Ⅰ组的IgM水平低于对照组(P <0 0 1 ) ,且IgM水平的高低与人参剂量呈正相关 (r =0 93,P <0 0 5) ;Ⅰ组的IgG2a水平高于对照组 (P <0 0 5) ;血清IgG2a水平与人参剂量呈正相关 (r =0 90 ,P <0 0 2 ) ;PA感染两周后 ,Ⅰ、Ⅱ、Ⅲ组的抗藻酸盐IgG水平高于对照组和Ⅳ组 (P <0 0 5) ,而Ⅰ、Ⅲ、Ⅳ组的IgA则低于对照组 (P <0 0 5)。结论 :2 5mg/kg的人参是本研究的最佳剂量 ,并显示了兴奋T辅助细胞Ⅰ型反应的效果
Objective :To investigate the effect of ginseng water extract (referred to as ginseng liquid) on chronic P. aeruginosa (PA) pneumonia in rats. Methods : Four different doses of ginseng subcutaneous injection were used to observe the efficacy after two weeks of treatment. RESULTS: Four doses of Ginseng liquor significantly reduced the gross pulmonary lesions and lung abscess rate (P < 0.05), and the degree of lesions were negatively correlated with the dose of ginseng (r=-0 95, P < 0 0 1 ) ; The number of mast cells in the ginseng I (5mg/kg), II (25mg/kg) and IV (065.2mg/kg) groups was lower than the control group (P < 0.05); II, III Pulmonary bacterial clearance in the (1 25 mg/kg) group was stronger than in the control group (P < 0.05); PA-specific antibody responses in the II, III, and IV groups were down regulated (P <0 01); group I. The level of IgM was lower than that of the control group (P < 0 01), and the level of IgM was positively correlated with the ginseng dose (r = 93, P <0 05); the IgG2a level of group I was higher than that of the control group (P < 0 0 5) ; Serum IgG2a levels were positively correlated with ginseng dose (r = 0 90, P <0 0 2); PA infection two weeks later, I, II, III group of anti-alginate IgG levels were higher than the control group and In group IV (P < 0.05), IgA in group I, III, IV was lower than that in control group (P <0 05). Conclusion :2 5 mg/kg of ginseng is the best dose in this study and shows the effect of type I response in T helper cells.