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目的观察脂多糖致幼鼠感染性脑水肿后脑组织β1整合素和基质金属蛋白酶2(MMP-2)表达的变化及其相关性研究。方法将50只幼鼠随机分为内毒素组(40只)和对照组(10只)。建立脂多糖致幼鼠感染性脑水肿模型。于术后6、12,24、48 h处死动物,分别行脑组织含水量,血脑脊液屏障(BBB)通透率测定。干湿法测脑组织含水量,以伊文思兰含量判定血脑屏障通透率,并应用免疫组织化学技术检测脑组织内β1整合素和MMP-2表达水平。结果内毒素组脑组织含水量和伊文思兰(EB)含量明显高于对照组。在脂多糖注射后6 h时,幼鼠脑组织β1整合素表达下降,且随时间延长及脑损伤的加重其表达呈持续下降趋势,各时间点与对照组比较均有显著性差异(P<0.01)。MMP-2表达明显增加,24h时达高峰,各时间点与对照组比较均有显著性差异(P<0.01)。MMP-2与脑组织含水量以及EB呈正相关;β1整合素和MMP-2表达呈负相关。结论β1整合素、MMP-2参与感染性脑水肿的发生发展,二者之间可能存在一定的调控。
Objective To observe the changes of the expression of β1 integrin and matrix metalloproteinase-2 (MMP-2) in brain and the correlation between the expression of lipopolysaccharide and encephalatrophy in rats. Methods Fifty young rats were randomly divided into endotoxin group (n = 40) and control group (n = 10). Establishment of a rat model of infectious brain edema induced by lipopolysaccharide. The animals were sacrificed at 6, 12, 24 and 48 hours after operation, and the brain water content and permeability of the blood-brain barrier (BBB) were measured respectively. The water content of brain tissue was measured by wet and dry method. The permeability of blood-brain barrier was judged by Evans blue content. The expression of β1 integrin and MMP-2 in brain tissue were detected by immunohistochemistry. Results The brain water content and the content of Evans blue (EB) in endotoxin group were significantly higher than those in control group. At 6 h after lipopolysaccharide injection, the expression of β1 integrin in brain tissue of young rats decreased, and the expression ofβ1 integrin decreased gradually with the increase of time and brain injury. There was a significant difference at each time point compared with the control group (P < 0.01). The expression of MMP-2 increased significantly and peaked at 24 hours. There was a significant difference between the two groups at each time point (P <0.01). There was a positive correlation between MMP-2 and brain water content and EB, while β1 integrin and MMP-2 were negatively correlated. Conclusion β1 integrin and MMP-2 are involved in the development of infectious brain edema. There may be some regulation between the two.