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目的观察芦根乙醇提取物对链脲佐菌素(streptozocin,STZ)诱导的糖尿病小鼠肾脏结构以及肾组织单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)与转化生长因子-β1(transforming?growth?factor-β1,TGF-β1)变化的影响。方法 STZ诱导制备糖尿病小鼠模型随机分为模型对照组、芦根高剂量组和芦根低剂量组,每组10只,另取10只小鼠作为正常对照组。各组灌胃饲养5w后,过碘酸-雪弗氏(periodic acid Schiff reaction,PAS)染色法检测各组小鼠肾脏结构,采用蛋白质免疫印迹法(Western-blot)法检测各组实验小鼠肾组织的MCP-1与TGF-β1的表达。结果PAS染色后,芦根高剂量组肾脏结构明显改善,接近正常对照组;而芦根低剂量组肾脏结构改变轻微,与模型对照组相似;Westen-blot检测后,MCP-1与TGF-β1呈现相似的变化,模型对照组高于正常对照组P<0.01,模型对照组高于高剂量组,P<0.05;高剂量组高于正常对照组,P<0.05;低剂量组高于正常对照组,P<0.01;低剂量组高于高剂量组,P<0.05。结论芦根乙醇提取物对STZ诱导糖尿病小鼠具有肾脏保护作用,其机制可能与抑制MCP-1-TGF-β1通路有关。
Objective To observe the effects of ethanol extract from Rehmannia glutinosa root on the changes of renal structure and the expression of monocyte chemoattractant protein-1 (MCP-1) and the growth of kidney in diabetic mice induced by streptozocin (STZ) Factor-β1 (transforming growth factor-β1, TGF-β1) changes. Methods The model of STZ-induced diabetic mice was randomly divided into model control group, high-dose rehmannia root group and low-dose rehmannia root group, with 10 mice in each group and 10 mice as normal control group. The rats in each group were fed with gavage for 5 weeks, and the renal structure of mice in each group was detected by periodic acid Schiff reaction (PAS) staining. Western blotting was used to detect the changes of renal structure in each group Renal Tissue MCP-1 and TGF-β1 Expression. Results After renal tissue was treated with PAS, the kidney structure of high dose rhubarb group was significantly improved, which was close to that of normal control group. The renal structure of low dose rhubarb group changed slightly, which was similar to that of model control group. (P <0.01), the model control group was higher than the high dose group, P <0.05; the high dose group was higher than the normal control group, P <0.05; the low dose group was higher than the normal control group Group, P <0.01; low dose group was higher than high dose group, P <0.05. Conclusion The ethanol extract of reed rhizome has renal protective effect on STZ-induced diabetic mice, which may be related to the inhibition of MCP-1-TGF-β1 pathway.