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OBJECTIVE To evaluate antioxidant,anti-inflammatory,antimicrobial activities of Pinus Roxburghii stem bark in vitro and evaluation of in vivo anti-osteoporotic activity in female rats.METHODS The antioxidant activity of plant extract was assessed using DPPH,nitric oxide and H2O2 radical scavenging methods.In vitro anti-inflammatory activity was evaluated using albumin denaturation,membrane stabilization methods at various concentrations.The antibacterial and anti-fungal activity of extracts was also done against micro-organisms:Pseudomonas aurignosa,Escherichia coli,Staphylococcus aureus,Bacillus subtilis and Candida albicans.Quantitative analysis of total phenolics,flavonoids and tannins were also estimated.Female Wistar albino rats(220-260g)were employed and ovaries were surgically removed bilaterally in anaesthesia which causes post-menopausal syndrome in all the groups except sham operated rats.Extracts of Pinus roxburghii stem at 100 and 200mg·kg-1,po.and tamoxifen as standard 1mg·kg-1,po.were given.Acute toxicity study was also done.RESULTS Treatment with Pinus roxburghii extract in two doses significantly(P<0.05)ameliorated surgical ovariectomy induced biochemical and biomechanical changes like decrease in bone density,bone strength,alkaline phosphatase,serum calcium,serum phosphorous and increase in urine hydroxylproline levels.Plant extract also has significant(P<0.05)antioxidant,antimicrobial and anti-inflammatory activities.CONCLUSION The assessments done in present investigation may conclude that the constituents of Pinus roxburghii has significant potential in ameliorating oxidative,inflammatory reactions and possesses anti-microbial;osteobalstic and osteoclastic activities used for calcium homeostatis and improved bone strength in female rats.
OBJECTIVE To evaluate antioxidant, anti-inflammatory, antimicrobial activities of Pinus Roxburghii stem bark in vitro and evaluation of in vivo anti-osteoporotic activity in female rats. METHODS The antioxidant activity of plant extract was assessed using DPPH, nitric oxide and H2O2 radical scavenging methods In vitro anti-inflammatory activity was evaluated using albumin denaturation, membrane stabilization methods at various concentrations. Antibacterial and anti-fungal activity of extracts was also done against micro-organisms: Pseudomonas aurignosa, Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida Quantitative analysis of total phenolics, flavonoids and tannins were also estimated. Male Wistar albino rats (220-260g) were employed and ovaries were surgically removed bilaterally in anaesthesia which causes post-menopausal syndrome in all the groups except sham operated rats. Extracts of Pinus roxburghii stem at 100 and 200 mg · kg-1, po.and tamoxifen as standard 1m g · kg -1, po.were given. Acute toxicity study was also done .RESULTS Treatment with Pinus roxburghii extract in two doses significantly (P <0.05) ameliorated surgical ovariectomy induced biochemical and biomechanical changes like decrease in bone density, bone strength, alkaline phosphatase, serum calcium, serum phosphorous and increase in urine hydroxylproline levels. Plant extract also has significant (P <0.05) antioxidant, antimicrobial and anti-inflammatory activities. CONCLUSION The assessments done in present investigation may conclude that the constituents of Pinus roxburghii has significant potential in ameliorating oxidative, inflammatory reactions and possesses anti-microbial; osteobalstic and osteoclastic activities used for calcium homeostatis and improved bone strength in female rats.