论文部分内容阅读
目的研究兔眼薄壁滤过泡形成后局部滴用左氧氟沙星滴眼液晶状体内浓度的变化。设计实验研究。研究对象新西兰白兔。方法将右眼行联合丝裂霉素C的巩膜咬切术后形成苍白、隆起、薄壁滤过泡的新西兰白兔26只,随机分为A、B、C三组。其中A、B组各8只,每日4次双眼滴左氧氟沙星滴眼液,每次0.1ml,分别持续4天和7天;C组10只,每30分钟双眼滴左氧氟沙星滴眼液一次,共点8次。三组全部最后一次滴左氧氟沙星滴眼液半小时后处死兔,取出晶状体,通过高效液相法(HPLC)检测晶状体中左氧氟沙星的浓度,分别比较各组中薄壁滤过泡眼与自身健康对照眼晶状体左氧氟沙星浓度的差别,比较各组之间薄壁滤过泡眼晶状体左氧氟沙星浓度的差别。主要指标兔眼晶状体中左氧氟沙星浓度。结果三组中薄壁滤过泡眼(A组:0.33±0.10μg/g;B组:0.27±0.15μg/g;C组:0.62±0.28μg/g)与自身健康对照眼(A组:0.12±0.03μg/g;B组:0.12±0.03μg/g; C组:0.15±0.05μg/g)晶状体中左氧氟沙星浓度差异有统计学意义(A组:P<0.0001;B组:P<0.05;C组:P<0.0001),A组和B组薄壁滤过泡眼晶状体左氧氟沙星浓度差异无统计学意义(P=0.566),A、B组与C组薄壁滤过泡眼晶状体左氧氟沙星浓度差异有统计学意义(P=0.006;0.001),C组(0.62±0.28μg/g)薄壁滤过泡眼晶状体中左氧氟沙星浓度分别高于A组(0.33±0.10μg/g)和B组(0.27±0.15μg/g)。薄壁滤过泡结膜下大片疏松区域,结膜上皮萎缩变薄,结膜基底层细胞不规则,间隙增宽,炎症细胞浸润。结论薄壁滤过泡形成后,眼内晶状体中左氧氟沙星浓度增高,频繁点药晶状体内左氧氟沙星浓度增高更为明显。
Objective To study the changes of concentration of levofloxacin drops in eye drops after the formation of thin-wall filter blebs in rabbit eyes. Design experiment research. New Zealand white rabbits Methods A total of 26 New Zealand white rabbits with pale, bulge and thin-wall bleb after scleral buckling of the right eye with mitomycin C were randomly divided into A, B and C groups. Eight of them were in group A and group B, and four times a day, two drops of levofloxacin eye drops, 0.1ml each time, lasting for 4 days and 7 days respectively. Group C, 10 eyes were dripped with levofloxacin once every 30 minutes for a total of Click 8 times. The rabbits were killed after three hours and three hours after the last dose of levofloxacin drops. The lenses were removed and the concentration of levofloxacin in the lens was determined by HPLC. The differences between the thin-wall filtered eyes and the healthy controls Lens levofloxacin concentrations were compared between the various groups thin-walled filtration eye lens levofloxacin concentration differences. Main indicators Rabbit eye levofloxacin concentration in the lens. Results Thin-walled bleb (group A: 0.33 ± 0.10 μg / g; group B: 0.27 ± 0.15 μg / g; group C: 0.62 ± 0.28 μg / g) 0.12 ± 0.03μg / g in group B; 0.12 ± 0.03μg / g in group B; 0.15 ± 0.05μg / g in group C) There was significant difference in the concentration of levofloxacin in the lens (group A: P <0.0001; ; Group C: P <0.0001). There was no significant difference in the concentrations of levofloxacin between group A and group B (P = 0.566), while the concentration of levofloxacin in group A, group B and group C The difference was statistically significant (P = 0.006; 0.001), levofloxacin in group C (0.62 ± 0.28μg / g) was significantly higher than that in group A (0.33 ± 0.10μg / g) and group B 0.27 ± 0.15 μg / g). Thin-walled filtration bleb conjunctival loose large area, the conjunctival epithelium atrophy thinning, irregular conjunctival basal cells, widening gap, inflammatory cell infiltration. Conclusion After the formation of thin-wall filtration bleb, the concentration of levofloxacin in the intraocular lens increased, and the concentration of levofloxacin in the intraocular lens increased more obviously.