论文部分内容阅读
目的 :观察小儿肾病综合征 (NS)强的松治疗前后激素耐药者外周血单个核细胞 (PBMC)的细胞凋亡变化 ,探讨PBMC细胞凋亡在激素治疗中的意义及与疗效之间的关系。方法 :1 5例激素耐药型肾病综合征患者分别于强的松治疗前和治疗后第 2、4、6、8周采血 ,进行细胞凋亡形态观察 ,计算百分率。结果 :治疗前与治疗后 2、4、6周比较均 P<0 .0 5差异有显著性意义 ;但第 8周尿蛋白未见转阴 ,而凋亡率下降。结论 :小儿 NS耐药型患者使用足量强的松治疗可短期诱导外周血 PBMC细胞凋亡增加 ,至 8周时诱导凋亡减弱 ,故持续足量使用不应超过八周。当尿蛋白未好转 ,应及时加用其它免疫抑制剂治疗 ,由此可见 NS激素治疗的疗效与外周血单个核细胞的细胞凋亡变化存在有一定关系
OBJECTIVE: To observe the changes of apoptosis of peripheral blood mononuclear cells (PBMCs) in hormone-resistant human children with nephrotic syndrome (NS) before and after treatment with prednisone, and to explore the significance of the apoptosis of PBMCs in hormone therapy and its therapeutic effect relationship. Methods: Fifteen patients with steroid-resistant nephrotic syndrome were collected before prednisone and 2, 4, 6, and 8 weeks after treatment respectively for morphological observation of apoptosis and calculation of percentage. Results: Before treatment and after 2, 4, and 6 weeks of treatment, there was a significant difference between the two groups (P <0.05). However, the urine protein in the eighth week did not turn negative while the apoptosis rate decreased. Conclusion: The use of prednisone in children with NS-resistant type can increase the apoptosis of peripheral blood PBMCs in a short term and lead to the decrease of apoptosis at 8 weeks. Therefore, it should not be used for more than eight weeks. When urinary protein is not improved, should be promptly added with other immunosuppressive agents, we can see the effect of NS hormone therapy and peripheral blood mononuclear cell apoptosis changes have a certain relationship