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通过GMA半薄切片技术对APⅣ不同类型水稻(Oryza sativa L.)胚囊的受精及其胚胎发育的研究表明,APⅣ中5-2-l型胚囊的3个卵细胞在少数情况下都可受精并发育形成3个胚;但多数情况只有 1个或2个卵细胞受精发育成1个胚或2个胚。6-2-0型和5-3-0型胚囊多个卵受精频率都很低。由此证明APIV多胚是来自如5-2-1型胚囊的多卵卵器胚囊多个卵细胞都受精的结果,其中3胚来自3个卵细胞受精发育,2胚来自2个卵细胞受精发育。双套结构胚囊受精最为复杂,多数情况是受精不正常,只有少数子房大、小胚囊中的卵细胞都能正常受精。大胚囊中的卵细胞受精发育可能是形成所谓中位胚(远离珠孔端胚)的主要原因。
Studies on the fertilization and embryo development of the embryo sacs of different APIV types of rice (Oryza sativa L.) by the GMA semi-thin section technique showed that in the few cases, all the three egg cells of the 5-2-1 embryo sac in APIV could be fertilized And develop to form three embryos; but in most cases only one or two eggs fertilized into one embryo or two embryos. Females of 6-2-0 and 5-3-0 blastocysts have low frequency of fertilization. Thus it was demonstrated that APIV polyembryo is the result of fertilization of multiple oocytes from the multi-oocyte embryo sac of the 5-2-1 type embryo sac, of which 3 embryos are fertilized by 3 egg cells and 2 embryos are fertilized by 2 egg cells . Double sets of embryo sac fertilization is the most complex, in most cases is not normal fertilization, only a few large ovary, small embryo sac in the normal fertilization of eggs. The fertilization of egg cells in the large embryo sac may be the main reason for the formation of the so-called metaphase embryo (far from the terminal populus).