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本研究对商陆(Phytolacca acinosa)进行茎尖微茎尖培养与多倍体诱导研究。研究结果表明:适合商陆微茎尖生长的培养基为MS+6-BA 1.0 mg/L+IBA 0.02 mg/L;适合诱导商陆微茎尖生根的培养基为1/2MS+NAA 0.5 mg/L+IBA 1.0 mg/L,诱导率为100%。用0.3%的琼脂溶胶配制成秋水仙素浓度为0.2%的溶胶药剂连续处理微茎尖48 h,获得变异植株,表现为植株健壮,叶片皱缩增大,颜色加深,气孔数目增多、增大。压片法对变异植株根尖进行染色体鉴定,证明变异株为多倍体。
In this study, Phytolacca acinosa was used to study the shoot tip and polyploid induction. The results showed that the medium suitable for the growth of P. apuslandica was MS + 6-BA 1.0 mg / L + IBA 0.02 mg / L, and the medium suitable for inducing the rooting of P. pseudoacacia was 1/2 MS + NAA 0.5 mg / L + IBA 1.0 mg / L, the induction rate was 100%. With 0.3% agar sol formulated colchicine concentration of 0.2% of the sol agent continuous treatment of micro-shoots for 48 h, obtained mutant plants, showing robust plants, folds increased, darker color, the number of stomata increased, increased . The tablet method was used to identify the root tip of the mutant plant, which proved that the mutant strain was polyploid.