论文部分内容阅读
目的:研究阿魏酸(ferulic acid,FA)在缺氧条件下对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)增殖、迁移和管腔样结构形成的影响。方法:原代培养人脐静脉内皮细胞,在缺氧实验条件下,细胞被分为7组,即1个对照组和6个实验组。对照组采用1%酒精处理,实验组用不同浓度(1×10~(-8)、1×10~(-7)、1×10~(-6)、1×10~(-5)、1×10~(-4)及1×10~(-3) mol/L)的阿魏酸处理。分别采用MTS法、划痕法、Matrigel法分析不同浓度阿魏酸处理对人脐静脉内皮细胞的增殖、迁移和管腔样结构形成的影响。结果:缺氧条件下,浓度为1×10~(-6)~1×10~(-4)mol/L的阿魏酸处理能明显促进HUVECs的增殖(P<0.05),以1×10~(-5) mol/L处理的效果最好(P<0.01);与对照组相比,1×10~(-6)mol/L(P<0.05)、1×10~(-5) mol/L(P<0.01)及1×10~(-4) mol/L(P<0.01)阿魏酸处理均能明显促进HUVECs横向迁移,以1×10~(-5) mol/L处理迁移的细胞数量最多;1×10~(-8)~1×10~(-4) mol/L阿魏酸处理能不同程度地促进HUVECs管腔样结构的形成,以1×10~(-5) mol/L处理形成管腔样结构的数量最多(P<0.01)。结论:阿魏酸在缺氧条件下能促进人脐静脉内皮细胞的增殖、迁移和管腔样结构形成。
Objective: To investigate the effect of ferulic acid (FA) on proliferation, migration and luminal structure formation of human umbilical vein endothelial cells (HUVECs) under hypoxic conditions. Methods: Primary cultured human umbilical vein endothelial cells under hypoxic conditions, the cells were divided into 7 groups, namely a control group and 6 experimental groups. The control group was treated with 1% alcohol. The experimental group was treated with different concentrations (1 × 10 -8, 1 × 10 -7, 1 × 10 -6, 1 × 10 -5, 1 × 10 ~ (-4) and 1 × 10 ~ (-3) mol / L) ferulic acid treatment. The effects of different concentrations of ferulic acid treatment on the proliferation, migration and luminal structure formation of human umbilical vein endothelial cells were analyzed by MTS method, scratch method and Matrigel method respectively. Results: Under hypoxia conditions, ferulic acid treatment at a concentration of 1 × 10 -6 -6 × 10 -4 mol / L significantly promoted the proliferation of HUVECs (P <0.05) Compared with the control group, the effect of 1 × 10 -6 mol / L (P <0.05), 1 × 10 -5 (-5) mol / Treatment with 1 × 10 ~ (-4) mol / L ferulic acid (P <0.01) and 1 × 10 ~ (-5) mol / L ferulic acid could significantly promote the horizontal migration of HUVECs. 1 × 10 -8 -8 mol·L -1 ferulic acid could promote the formation of luminal-like structures in HUVECs to a certain extent, with 1 × 10 ~ (-1) 5) mol / L formed the largest number of lumen-like structures (P <0.01). CONCLUSION: Ferulic acid can promote the proliferation, migration and formation of lumen-like structure of human umbilical vein endothelial cells under hypoxic conditions.