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本工作用体外抗体产生系统,研究了由免疫核糖核酸(iRNA)引起的、参与免疫记忆的T细胞与B细胞.实验动物采用8-12周龄的BALB/C小鼠及由BALB/C小鼠培育的裸鼠.抗原用10%绵羊红细胞(SRBC)或马红细胞(HRBC).取0.1ml抗原给小鼠作腹腔内注射,四周后再加强注射.免疫后五天将小鼠解剖,用改进Kidson法自小鼠脾脏提取iRNA.取500μgiRNA给小鼠作腹腔注射,四周后再加强注射.脾细胞的制备及试管内抗体形成的测定:在双玻皿内将小鼠脾脏切开,加入培养液制备成无菌的单个细胞悬液.在1ml RPMl1640培养液内计数脾细胞1×10~7,并加入青霉素G50U/ml、硫酸链霉素50μg/ml、硫烷醇(5×10~(-5)M),再补足20%胎
This work uses an in vitro antibody production system to study the immunological memory of T cells and B cells caused by the immunoreactive ribonucleic acid (iRNA) .The BALB / C mice aged 8-12 weeks and BALB / C mice The nude mice were incubated with 10% SRBC or HRBC.The mice were injected intraperitoneally with 0.1 ml of antigen and injected four more weeks later.The mice were dissected five days after the immunization, Improve the method of Kidson from the mouse spleen extract iRNA.Apply 500μ g RNA to mice for intraperitoneal injection, and then strengthen the injection after four weeks.Preparation of spleen cells and in vitro antibody formation in the mouse: the spleen was cut in double glass dish, add The culture medium was prepared into a sterile single cell suspension in 1ml RPMl1640 culture medium count spleen cells 1 × 10 ~ 7, and added penicillin G50U / ml, streptomycin 50μg / ml, thiolanes (5 × 10 ~ (-5) M), then make up 20% of the tire