论文部分内容阅读
体外中性白细胞(PMN)趋化试验是测定白细胞功能的一种方法。用琼脂糖凝胶技术和不连续梯度细胞分离液一次分离PMN,使该方法简化并能较广泛地应用于临床。现将本室开展此项工作结果报道于后。 一、材料和方法:根据Nelson和张成武报告加以修改。 (一)细胞制备参照Aguado氏法用1.077和1.097比重的聚蔗糖-泛影葡胺不连续的密度梯度方法一次性分离,获得PMN悬液。 (二)趋化因子为24小时培养的大肠杆菌悬液。
In vitro neutrophil (PMN) chemotaxis assay is a method of determining leukocyte function. The separation of PMN by agarose gel technology and discontinuous gradient cell separation liquid makes the method simple and can be widely used in clinic. Now this room to carry out the results of this work reported later. First, materials and methods: According to Nelson and Zhang Chengwu report to be modified. (A) Cell Preparation According to the Aguado’s method, the PMN suspension was obtained by one-time separation using the density gradient method of Ficoll-Diatrizoate with a specific gravity of 1.077 and 1.097. (B) Chemotactic factors for 24-hour culture of E. coli suspension.