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建立了反相银化高效液相色谱测定银杏叶中银杏酚酸含量的分析方法。在样品的浸提液中加入少量酸性盐溶液和吸附剂后 ,用一步反萃取法净化样品 ,有机相浓缩后供HPLC分析。流动相 :V(甲醇 )∶V(体积分数为 5 %的乙酸水溶液 ) =90∶10 ,其中银离子浓度 0 0 3mol·L-1,紫外检测波长 310nm。结果表明 4种银杏酚酸之间达到基线分离 ,该方法平均回收率为97 3% ,相对标准偏差 1 6 % ,最低检测量 0 0 2 6 μg ,可有效地用于银杏叶及其提取物中银杏酚酸的定量分析。
A method for the determination of ginkgolic acids in Ginkgo biloba leaves by reversed-phase high performance liquid chromatography was established. After adding a small amount of acidic salt solution and adsorbent to the sample extract, the sample was purified by one step back extraction and the organic phase was concentrated for HPLC analysis. Mobile phase: V (methanol): V (volume fraction of 5% acetic acid in water) = 90:10, silver ion concentration of 0 0 3mol·L-1, UV detection wavelength 310nm. The results showed that the four kinds of ginkgolic acids to achieve baseline separation, the average recovery of 97 3%, the relative standard deviation of 16%, the lowest detection of 0 0 2 6 μg, can be effectively used for Ginkgo biloba and its extracts Quantitative Analysis of Ginkgolic Acid.