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探讨 ＥＬＩＳＡ一步法检测两种不同乙型肝炎血清学模式标本的 ＨＢｓＡｇ吸光度值差异的原因。 方法：将同一时间内 ＥＬＩＳＡ一步法检测的所有 ＨＢａＡｇ阳性标本分为 ＨＢｅＡｇ阳性与 ＨＢｅＡｇ阴性两种血清学模 式，对其原血清及１∶１０稀释血清的ＨＢｓＡｇ吸光度值进行比较。结果：当用原血清做ＥＬＩＳＡ一步法试验时， ＨＢｓＡｇ、ＨＢｅＡｇ均阳性标本的ＨＢｓＡｇ吸光度值显著低于ＨＢｓＡｇ阳性、ＨＢｅＡｇ阴性标本，而将标本进行１∶１０稀 释后进行同步对比试验，则发现第一种血清学模式标本 ＨＢｓＡｇ吸光度值显著高于第二种模式标本 ＨＢｓＡｇ吸 光度值。结论：与ＨＢｅＡｇ阴性标本比较，ＨＢｅＡｇ阳性标本的ＨＢｓＡｇ滴度相对较高，而ＥＬＩＳＡ一步法检测高滴 度ＨＢｓＡｇ标本时，不可避免会出现显色淡，吸光度值偏低的结果，故应采用经典二步法操作。
To investigate the difference of HBsAg absorbance between two different serological models of hepatitis B by one-step ELISA. Methods: All the HBaAg positive samples detected by one-step ELISA in the same time were divided into two serological models: HBeAg-positive and HBeAg-negative. The HBsAg absorbance values of the original serum and 1:10 diluted serum were compared. Results: When using the original serum ELISA one-step ELISA test, HBsAg, HBeAg positive specimens HBsAg absorbance values were significantly lower than the HBsAg positive, HBeAg negative specimens, and the specimens were diluted 1:10 after a comparative test, then found that HBsAg absorbance value of a serological model specimen was significantly higher than that of the second mode specimen HBsAg absorbance value. Conclusion: Compared with HBeAg negative samples, HBeAg HBeAg positive samples HBsAg titer is relatively high, and ELISA one-step detection of high titer HBsAg specimens inevitably appear pale color, the value of the absorbance value is low, so the classic Two-step operation.