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利用高效毛细管法(HPCE)对人工倾斜的中林46杨(Populus×euramericana(Dode)Guiner CL.Zhonglin-46)树干形成层内源激素进行测定。结合自身试验材料的特殊性,优化样品前处理过程,选择符合自身材料的最佳电泳条件,探索出测定树干形成层5种内源激素赤霉素(GA3)、吲哚-3-乙酸(IAA)、吲哚-3-丁酸(IBA)、玉米素(Z)和脱落酸(ABA)的方法。最佳条件为:电压16 kV、柱温20℃、缓冲液pH 9.25、SDS浓度50mmo.lL-1。5种内源激素的相关系数R2为0.978 6~0.999 9,回收率为93%~105%,变异系数≤3.93%。
The endogenous phytohormones in the stem-forming layer of Populus × euramericana (Dode Guiner CL.Zhonglin-46) were measured by high performance capillary method (HPCE). Based on the particularity of the materials tested, the optimal pretreatment process was optimized and the best electrophoresis conditions were selected to explore the best method for the determination of five endogenous GA3, IAA ), Indole-3-butyric acid (IBA), zeatin (Z) and abscisic acid (ABA). The optimum conditions were as follows: the correlation coefficient R2 of voltage of 16 kV, column temperature 20 ℃, buffer pH 9.25, SDS concentration 50mmo.lL-1.5 endogenous hormones was 0.978 6-0.9999, the recovery was 93% -105 %, Coefficient of variation ≤3.93%.