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目的研究大鼠作为泡球蚴保种模型动物的可行性。方法选取Wistar、SD大鼠,使用在长爪沙鼠体内保种的泡球蚴组织碎片进行腹腔接种,并对接种成功鼠种进行种内二次传代和抗泡球蚴组织多克隆抗体的免疫组化鉴定。同时用新西兰白兔、豚鼠、长爪沙鼠建模,观察泡球蚴感染情况。结果新西兰白兔、豚鼠、未能通过腹腔接种感染,Wistar大鼠、SD大鼠获得感染,感染率分别为85%和80%,长爪沙鼠感染率100%;感染大鼠可有一个较长的存活期,体内可见到生长活跃的泡球蚴虫体组织和原头节;泡球蚴能在Wistar大鼠、SD大鼠中稳定传代。结论 Wistar和SD大鼠是较理想的泡球蚴保虫动物。
Objective To study the feasibility of using rat as a metacercariae preservation model animal. Methods Wistar and SD rats were selected for intraperitoneal inoculation using the fragments of metacercariae kept in the body of Mongolian gerbil (Meriones unguiculatus), and the mice were inoculated intraperitoneally for two passages and immunized with polyclonal antibodies to Cysticercus cellulosae Group identification. At the same time, New Zealand white rabbits, guinea pigs and Mongolian gerbils were used to model the infection of metacercariae. Results In New Zealand White rabbits and guinea pigs, infection in Wistar rats and Sprague-Dawley rats was not successful. The infection rates were 85% and 80%, respectively. The infection rate of gerbils was 100% Long survival period, the body can be seen in the active growth of Echinococcosis and protoscolema; Cysticercus can Wistar rats, SD rats stable passage. Conclusion Wistar and SD rats are the ideal cyperus larvae.