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为了解人参对造血祖细胞的作用,应用红系祖细胞(BFU-E、CFU-E)和粒单系祖细胞(CFU-GM)体外培养技术,观察人参总皂甙(TSPG)对正常人和29例再生障碍性贫血(再障)患者的祖细胞刺激增殖作用,并与甲基睾丸素(甲睾)相比较。TSPG在20μg/ml浓度时,可使正常BFU-E、CFU-E和CFU-GM产率分别提高37.8±2.9%、31.4±2.9%和33.4±4.0%。去除红细胞生成素,TSPG亦具有直接刺激红系祖细胞增殖作用,而甲睾对CFM-GM无作用。29例再障中雄激素反应型14例(48.1%),均同时呈现对人参的敏感性(集落数增加30%以上)。而免疫介导型及干细胞缺少型中人参的作用均不能表达。提示TSPG的作用机制可能是通过增强红细胞生成素等造血生长因子的活性间接地促进骨髓造血。
To understand the effect of ginseng on hematopoietic progenitor cells, erythroid progenitor cells (BFU-E, CFU-E) and myeloid progenitor cells (CFU-GM) were cultured in vitro to observe the effect of ginsenosides (TSPG) on normal people and Progenitor cells from 29 patients with aplastic anemia (aplastic anemia) stimulated proliferation and were compared with methyltestosterone (methyl testosterone). TSPG at a concentration of 20 μg/ml increased the yield of normal BFU-E, CFU-E, and CFU-GM by 37.8±2.9%, 31.4±2.9%, and 33.4±4.0%, respectively. With the elimination of erythropoietin, TSPG also has a direct effect on the proliferation of erythroid progenitor cells, whereas a testosterone has no effect on CFM-GM. Twenty-four cases (48.1%) of the androgen-responsive type in 29 cases of aplastic anemia all showed sensitivity to ginseng (the number of colonies increased by more than 30%). However, the effects of ginseng in immune-mediated and stem cell-deficient models could not be expressed. It is suggested that the mechanism of action of TSPG may be to indirectly promote bone marrow hematopoiesis by enhancing the activity of hematopoietic growth factors such as erythropoietin.