转基因大豆对雌鼠胚胎发育及受精能力的影响

• 作 者: 芦春斌 郑建新 蔡娟 隗洋洋 戚青林 刘标
• 机 构: 暨南大学生命科学技术学院,环境保护部南京环境科学研究所,
• 出 处: 大豆科学
• 发表日期: 2014年04期
• 关键词: 雌鼠 转基因大豆 动情周期 受精能力 胚胎发育 生殖毒理 受精卵 非转基因大豆 体内受精 囊胚

为评估转基因大豆对雌鼠胚胎发育及受精能力的影响,用转基因大豆饲料(试验组)与非转基因大豆饲料(对照组)分别喂食雌鼠,30和90 d后分别检测观察动情周期变化规律及受精卵、2-细胞胚胎、囊胚在体内的发育情况。结果显示:试验组与对照组雌鼠动情周期均在4~5 d范围内,无显著差异;与对照组相比,试验组雌鼠体内受精的合子(受精卵、2-细胞胚胎、囊胚)数量、受精率、优胚率无显著性差异。研究结果表明:短期(30 d)和长期(90 d)喂食转基因大豆不会对雌鼠动情周期、受精能力及早期胚胎发育发生不良影响。

To evaluate the effects of transgenic soybean on embryonic development and fertilization in female rats, female rats were fed with transgenic soybean (experimental group) and non-transgenic soybean (control group) respectively. The changes of estrous cycle were observed after 30 and 90 days, respectively. Fertilized eggs, 2-cell embryos, blastocysts in vivo development. The results showed that the estrous cycle of the test group and the control group were within 4 ~ 5 days, there was no significant difference; compared with the control group, fertilized zygotes (fertilized eggs, 2-cell embryos, blastocysts ) Number, fertilization rate, excellent embryo rate no significant difference. The results showed that the short-term (30 d) and long-term (90 d) feeding of transgenic soybean did not affect the estrus cycle, fertilization ability and early embryo development of female rats.