论文部分内容阅读
目的探索子痫前期患者syncytin2及其受体MFSD2在胎盘的表达情况以及缺氧对其表达的影响。方法采用荧光实时定量PCR方法检测轻度子痫前期组(15例)、重度子痫前期组(15例)、妊娠高血压组(10例)、子痫组(5例)及对照组(20例)胎盘组织中syncytin2及其受体MFSD2mRNA表达;通过Western blot法检测syncytin2及其受体MFSD2的蛋白表达情况,分析各组间的表达差异;比较正常和缺氧条件下forskolin诱导Be Wo细胞分化成合体滋养细胞时,syncytin2及其受体MFSD2表达的变化。结果 1病理组各组的syncytin2及其受体MFSD2的蛋白表达低于对照组,差异有统计学意义(P<0.01)。重度子痫前期组与病理组各组间子痫组的蛋白表达无统计学差异(P>0.05),妊娠期高血压组与轻度子痫前期表达无统计学差异(P>0.05)。随着子痫程度的加重,syncytin2的蛋白表达及受体MFSD2呈下降趋势。2在正常氧培养的状态下,对照组syncytin2的蛋白表达与受体MFSD2的mRNA处于低水平,在Forskolin作用48h之后,两者的syncytin2的mRNA表达明显增加(P<0.05),而MFSD2表达明显减少(P<0.05)。结论 syncytin2与受体MFSD2的蛋白表达与孕周存在着密切的相关性。缺氧导致syncytin2及其受体MFSD2表达异常与子痫前期合体滋养细胞异常有关。
Objective To explore the expression of syncytin2 and its receptor MFSD2 in the placenta and the effect of hypoxia on the expression of syncytin2 in patients with preeclampsia. Methods Fifteen cases of mild preeclampsia group, 15 cases of severe preeclampsia group, 10 cases of pregnancy induced hypertension group, 5 cases of eclampsia group and 20 cases of control group were detected by real-time fluorescence quantitative PCR. Cases) Placenta syncytin2 and its receptor MFSD2mRNA expression; By Western blot detection of syncytin2 and its receptor MFSD2 protein expression analysis of the differences between the groups; compared normal and hypoxic conditions forskolin-induced Be Wo cells differentiation Syncytin 2 and its receptor MFSD2 expression in syncytiotrophoblasts. Results 1 The protein expression of syncytin2 and its receptor MFSD2 in each pathological group was lower than that in the control group, the difference was statistically significant (P <0.01). There was no significant difference in the expression of protein in the eclampsia group between the severe preeclampsia group and the pathological group (P> 0.05). There was no significant difference between the preeclampsia group and the mild preeclampsia group (P> 0.05). As the degree of eclampsia increased, the protein expression of syncytin2 and the receptor MFSD2 showed a decreasing trend. The expression of syncytin2 in the control group was significantly lower than that of the MFSD2 mRNA in the normal control group (P <0.05), while the expression of syncytin2 was significantly increased in the control group (P <0.05) after 48h treatment with Forskolin Decrease (P <0.05). Conclusion There is a close correlation between protein expression of syncytin2 and receptor MFSD2 and gestational age. Hypoxia leads to syncytin2 and its receptor MFSD2 abnormal expression and preeclampsia syncytium trophoblast abnormalities.