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HB转录因子家族基因在植物生长、发育、生物和非生物胁迫中发挥着重要作用。根据已知序列Unigene551_All设计引物,利用SMARTer RACE技术从向日葵中克隆了一个HD-Zip类转录因子HB-12,该基因全长cDNA序列821 bp,包括长度为573 bp的开放阅读框,编码190个氨基酸。预测该蛋白的分子量和等电点分别为22.55 kD和5.58,具有一个同源异型框结构域(homeobox domain,HD)和一个同源异型框结合类亮氨酸拉链结构域(homeobox associated leucine zipper domain,HALZ),属于向日葵HD-ZipⅠ类蛋白,基因登录号为KU315052。HB-12蛋白不存在跨膜域,亚细胞定位预测其可能位于细胞核。聚类分析发现向日葵HB-12与茄科作物马铃薯和番茄HB-12基因亲缘关系较近。利用PCR技术扩增了HB-12全长cDNA对应的g DNA序列,该序列自起始密码子ATG至终止密码子TAA长度为652 bp,由2个外显子和1个内含子组成,基因登录号为KU315053。Real-time PCR结果表明,HB-12在向日葵根、下胚轴和叶中的表达存在器官特异性,且受盐、ABA及PEG的诱导表达。HB-12基因的克隆及表达分析为向日葵抗逆分子育种和功能研究奠定了基础。
HB transcription factor family genes play an important role in plant growth, development, biotic and abiotic stress. According to the known sequence Unigene551_All primers, an HD-Zip transcription factor HB-12 was cloned from sunflower by using SMARTer RACE technology. The full length cDNA sequence of this gene was 821 bp, including an open reading frame of 573 bp encoding 190 Amino acids. The molecular weight and isoelectric point of this protein were predicted to be 22.55 kD and 5.58, respectively, with a homeobox domain (HD) and a homeobox associated leucine zipper domain , HALZ) belongs to the sunflower HD-Zip class I protein, gene accession number KU315052. HB-12 does not exist transmembrane domain protein, subcellular localization predicted that it may be located in the nucleus. Cluster analysis found that sunflower HB-12 is closely related to solanaceous potato and tomato HB-12 genes. The g DNA sequence corresponding to the full-length cDNA of HB-12 was amplified by PCR. The sequence length from the start codon ATG to the stop codon TAA is 652 bp, which consists of two exons and one intron, Gene accession number KU315053. Real-time PCR results showed that the expression of HB-12 was organ-specific in roots, hypocotyls and leaves of sunflower and was induced by salt, ABA and PEG. The cloning and expression analysis of HB-12 gene laid the foundation for the research of sunflower anti-infective molecular breeding and function.