脂质体包裹EGFR反义寡脱氧核苷酸抑制人大肠癌细胞的增殖

• 作 者: 吴金生 何勇 王述民
• 机 构: 第四军医大学唐都医院普通外科
• 出 处: 华人消化杂志
• 发表日期: 1998年09期
• 关键词: 脂质体 包裹 反义寡脱氧核苷酸 人大肠癌

目的研究用表皮生长因子受体（ＥＧＦＲ）反义寡脱氧核苷酸（ＡＳＯＤＮ）抑制人大肠癌细胞株ＨＲ８３４８的生长增殖，探讨大肠癌治疗的新途径．方法人工合成的ＥＧＦＲ反义ＯＤＮ及无关对照ＯＤＮ经脂质体包裹后作用ＨＲ８３４８细胞，采用ＭＴＴ法，［３Ｈ］ＴｄＲ掺入试验，细胞周期分析，裸鼠体内接种等方法测定细胞体内外增殖的变化．结果经ＥＧＦＲ反义ＯＤＮ处理的ＨＲ８３４８细胞与对照组ＨＲ８３４８细胞相比，体外增殖速度减慢（细胞增殖抑制率达７１％）、ＤＮＡ合成受抑（细胞增殖指数为０３６１ｖｓ０７８４，Ｓ期细胞数为３４８％ｖｓ５９９％）、裸鼠体内成瘤率下降（２５％ｖｓ１００％）．结论ＥＧＦＲ反义ＯＤＮ可有效抑制大肠癌细胞的体内外生长增殖，可用于实验性肿瘤基因治疗研究

Objective To study the inhibition of growth and proliferation of human colorectal cancer cell line HR8348 by epidermal growth factor receptor (EGFR) antisense oligodeoxynucleotide (ASODN) and to explore a new approach for the treatment of colorectal cancer. Methods Synthesized EGFR antisense ODN and irrelevant control ODN were applied to HR8348 cells after liposome encapsulation. MTT assay, [3H] TdR incorporation assay, cell cycle analysis, and nude mice inoculation were used to determine the proliferation of cells in vitro and in vivo. The change. Results Compared with HR8348 cells in control group, HR8348 cells treated with EGFR antisense ODN slowed down in vitro (inhibition of cell proliferation was up to 71%) and DNA synthesis was inhibited (cell proliferation index was 0361 vs 0784, S phase). The number of cells was 348% vs599%) and the tumor formation rate in nude mice decreased (25% vs 100%). Conclusion EGFR antisense ODN can effectively inhibit the growth and proliferation of colorectal cancer cells in vivo and in vitro. It can be used for experimental tumor gene therapy research.