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AIM:To study the relationship between Survivin andPTEN expression and lymph node metastasis,depth ofinvasion and prognosis of gastric cancer patients in Chi-na.METHODS:Specimens of gastric cancer tissue were col-lected from the Affiliated Hospital of Jianghan University.All the 140 patients had complete examination data.Alllymph nodes were found by the fat-clearing method.Theinterrupted serial 4-micron sections,routine hematoxylinand eosin staining and immunohistochemical methodswere used to detect the lymph node metastases.Gastriccancer tissue microarray was performed to test the ex-pression of Survivin and PTEN(17A)in gastric cancer byimmunohistochemical method.All data were processedusing x~2 test,Fisher’s exact test,Kaplan-Meyer Log-rankmethod and Cox multivariate analysis(SPSS 12.0 soft-ware).RESULTS:One hundred and eighteen specimenswere used in our tissue microarray(utilization rate was82.4%).A total of 7580 lymph nodes were found.Metas-tases were found in 90 specimens and 1618 lymph nodeswere detected.The positive rate of Survivin and PTENexpression was 52.5%(62/118)and 76.2%(90/118),respectively.A highly positive correlation was found be-tween Survivin and PTEN expression(x~2=4.17,P=0.04).Survivin expression was positively correlated with UICCN stage(x~2=8.69,P=0.03)and histological classifica- tion(x~2=4.41,P=0.04)by x~2 tests.PTEN expression waspositively correlated with depth of invasion(P=0.02)andhistological classification(x~2=5.47,P=0.02).But Survivinand PTEN expressions were not related with prognosis ofgastric cancer patients.A significant correlation betweenlymph node metastasis and prognosis was demonstratedby Cox multivariate analysis(x~2=4.85,P=0.028).CONCLUSION:Survivin is positively correlated withPTEN expression in gastric cancer and is a molecularmarker of lymph node metastasis while PTEN expressionis a molecular marker of advanced gastric cancer.UICCN stage is the most important prognostic factor of gastriccancer in China.
AIM:To study the relationship between Survivin andPTEN expression and lymph node metastasis,depth ofinvasion and prognosis of gastric cancer patients in Chi-na.METHODS:Specimens of gastric cancer tissue were col-lected from the Affiliated Hospital of Jianghan University.All the 140 Patients had complete examination data.All lymph nodes were found by the fat-clearing method.The interrupted serial 4-micron sections,routine hematoxylinand eosin staining and immunohistochemical methodswere used to detect the lymph node metastases.Gastriccancer tissue microarray was performed to test the ex-pression Of Survivin and PTEN(17A)in gastric cancer byimmunohistochemical method.All data were processedusing x~2 test,Fisher’s exact test,Kaplan-Meyer Log-rankmethod and Cox multivariate analysis(SPSS 12.0 soft-ware).RESULTS:One hundred and eighteen Specimenswere used in our tissue microarray(utilization rate was82.4%).A total of 7580 lymph nodes were found.Metas-tases were found in 90 specimens and 1618 Lymph nodeswere detected.The positive rate of Survivin and PTENexpression was 52.5%(62/118)and 76.2%(90/118),respectively.Astrong positive correlation was found be-tween Survivin and PTEN expression(x~2=4.17, P = 0.04). Survivin expression was positively correlated with UICCN stage (x~2=8.69, P=0.03) and histological expression (x~2=4.41, P=0.04) by x~2 tests. PTEN expression was positively correlated. With depth of invasion (P=0.02) and histological classification (x~2=5.47, P=0.02). But Survivin and PTEN expressions were not not related with prognosis of gastric cancer patients. A significant correlation between lymph node metastasis and prognosis was demonstrated by Cox multivariate analysis ( x~2=4.85,P=0.028)CONCLUSION:Survivin is positively correlated withPTEN expression in gastric cancer and is a molecularmarker of lymph node metastasis while PTEN expressionis a molecular marker of advanced gastric cancer.UICCN stage is the most important prognostic factor of Gastriccancer in China.