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A spectrofluorimetric method for the determining riboflavin (VB 2 ) based on its enhancement on the fluorescence of hemoglobin|catalyzed enzymatic reaction was proposed. The proposed method consisted of two reactions. One was the photochemical reaction of VB 2 , the other was a hemoglobin|catalyzed enzymatic reaction. The optimal experimental conditions for the determinations were established. The linear range of the method was 5.0×10 -9 |1.0×10 -7 mol/L of VB 2 . The detection limit was calculated to be 3.65×10 -9 mol/L. The relative standard deviation of this method was 2.3 % at 7.0×10 -8 mol/L for 11 determinations.
A spectrofluorimetric method for determining riboflavin (VB 2) based on its enhancement on the fluorescence of hemoglobin | catalyzed enzymatic reaction was proposed. The proposed method consisted of two reactions. One was the photochemical reaction of VB 2, the other was a The optimal experimental conditions for the determinations were established. The linear range of the method was 5.0 × 10 -9 | 1.0 × 10 -7 mol / L of VB 2. The detection limit was to be 3.65 × 10 -9 mol / L The relative standard deviation of this method was 2.3% at 7.0 × 10 -8 mol / L for 11 determinations.