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目的:探讨IL-18基因启动子-607 C/A位点多态性与川崎病(KD)的关系。方法:收集50例KD患儿(KD组)和50例健康儿童(正常组)资料,采集血液标本,分离血浆和有核细胞,用SSP-PCR法检测IL-18基因-607位点基因型、酶联免疫吸附法测定血浆IL-18含量以及免疫比浊法测定血浆CRP含量。结果:KD组患儿-607 C/A位CC基因型频率为42.0%,高于正常组儿童(P<0.05);CC基因型KD患儿血浆IL-18含量显著高于CA基因型患者(P<0.01),前者血浆CRP含量也高于后者(P<0.05)。结论:IL-18基因启动子-607 C/A位点多态性参与KD发病,IL-18基因-607位点基因型的检测可用于预测KD发病和预后判断。
Objective: To investigate the relationship between the polymorphism of -607 C / A locus in IL-18 gene and Kawasaki disease (KD). Methods: Fifty KD children (KD group) and 50 healthy children (normal group) were collected. Blood samples were taken and plasma and nucleated cells were separated. The genotype of IL-18 gene at site -607 was detected by SSP-PCR , The content of plasma IL-18 by enzyme-linked immunosorbent assay, and the plasma CRP level by immunoturbidimetry. Results: The frequency of CC genotype at -607 C / A in KD patients was 42.0%, higher than that in normal children (P <0.05). The plasma IL-18 levels in children with CC genotype KD were significantly higher than those in CA genotypes P <0.01), the former plasma CRP level is also higher than the latter (P <0.05). CONCLUSION: Polymorphism of -607 C / A locus in IL-18 gene promoter is involved in the pathogenesis of KD. The genotype of IL-18 gene at locus -607 may be used to predict the onset and prognosis of KD.