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目的改进现有我国职业卫生标准方法中工作场所空气中臭氧的分光光度检测方法。方法工作场所空气中臭氧以0.5 L/min流量经10 ml碱性吸收液采集30 min,然后酸化析出碘,再用淀粉试剂显色比色测定。根据颜色深浅,比色定量。空气中还原性干扰物质用三氧化铬氧化管除去。在540 nm波长下测量吸光度,进行定量。结果方法的测定范围在0.5μg~6μg;标准曲线回归方程为y=-0.0017+0.0912x,相关系数r=0.9996;本法的检出限为0.05μg/ml;最小检出浓度为0.05 mg/m3(以15 L计)。样品室温下至少保存3 d。结论与现有的我国职业卫生标准方法相比,改进的方法简便、快捷、稳定、易于操作,方法的各项指标:线性关系、精密度、样品稳定性等,经实验验证均符合《工作场所空气中有毒物质检测方法研制规范》的要求,方法适用于工作场所空气中臭氧浓度的测定。
Objective To improve the spectrophotometric method for the determination of ozone in workplace air in the existing methods of occupational health standards in our country. Methods Ozone in the air of the workplace was collected for 30 min at a flow rate of 0.5 L / min through 10 ml of alkaline absorption solution, then acidified to precipitate iodine, which was then assayed by colorimetric determination of starch reagent. According to the color depth, colorimetric. Reducing airborne interfering substances with chromium oxide oxidation tube to remove. Absorbance was measured at 540 nm and quantitated. Results The calibration curve ranged from 0.5μg to 6μg. The regression equation of standard curve was y = -0.0017 + 0.0912x, the correlation coefficient was 0.9996. The detection limit was 0.05μg / ml. The minimum detectable concentration was 0.05 mg / m3 (based on 15 L). Samples are kept for at least 3 days at room temperature. Conclusion Compared with the existing methods of occupational health standards in our country, the improved method is simple, rapid, stable and easy to operate. The indexes of the method, such as linear relationship, precision and stability of samples, are validated in the workplace Air toxic substances test method for the development of norms "requirement, the method is suitable for the determination of the concentration of ozone in the workplace air.