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Pseudomonas fluorescens CPF-10和2P24分离自小麦全蚀病自然衰退土壤,两者都产生酚类抗生素2,4-二乙酰基间苯三酚(2,4-diacetylphloroglucinol,2,4-DAPG),对多种土传植物病害具有较好的防治能力.利用Tn5转座子携带2,4-DAPG合成基因簇分别整合到野生荧光假单胞菌P32(2,4-DAPG-),CPF-10和2P24的染色体上,HPLC结果表明2,4-DAPG阴性菌P32可以产生抗生素2,4-DAPG,2,4-DAPG阳性菌CPF-10和2P24的抗生素产量可显著提高.番茄青枯病菌、小麦全蚀病菌、棉花立枯病菌的平板抑菌实验和番茄青枯病、小麦全蚀病温室防病实验结果说明2,4-DAPG产量的提高对防治病害具有增效作用.工程菌与野生菌株在灭菌土中定殖能力没有显著差异,但在自然土中工程菌较野生菌的定殖能力显著提高.结果表明,提高抗生素2,4-DAPG的产量是提高荧光假单胞菌防治病害效果的有效途径之一.
Pseudomonas fluorescens CPF-10 and 2P24 were isolated from naturally eroded soils of wheat eclipses and both produced 2,4-diacetylphloroglucinol (2,4-DAPG), a phenolic antibiotic, A variety of soil-transmitted plant diseases have better prevention and treatment ability.Using the Tn5 transposon carrying 2,4-DAPG synthetic gene cluster were integrated into Pseudomonas fluorescens P32 (2,4-DAPG -), CPF-10 and 2P24, the results of HPLC showed that the antibiotic 2,4-DAPG could be produced by 2,4-DAPG-negative bacteria P32 and the yield of 2,4-DAPG-positive bacteria CPF-10 and 2P24 could be significantly increased. The results showed that the increase of 2,4-DAPG had a synergistic effect on the prevention and treatment of disease, and that of engineering bacteria and wild strains The ability of colonization in sterilized soil was not significantly different, but the colonization ability of engineered bacteria in natural soil was significantly higher than that of wild-type bacteria.The results showed that increasing the yield of 2,4-DAPG antibiotic is to improve the prevention and treatment of disease caused by Pseudomonas fluorescens One of the effective ways to effect.