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应用原子力显微镜分析内耳螺旋器(Corti器)不同部位的弹性特征。采用豚鼠内耳基底膜底回新鲜标本,用原子力显微镜在液相接触式测量,获得不同部位力曲线。经计算,对应Corti器相当于Hensen细胞、外毛细胞、柱细胞、内毛细胞、内指细胞、盖膜的部位及基底膜底面局部,其杨氏模量均值分别为46±1.7、59±0.9、250±31、140±2.8、430±29.9、210±7.2和230±8.8kPa。结果表明,基底膜径向排列的组织结构不同,杨氏模量存在明显差异,在整块基底膜标本上测量Corti器各结构的杨氏模量能更准确地反映它们在生理状态下的弹性特征。
Atomic force microscope was used to analyze the elastic characteristics of different parts of the Corti. Guinea pig inner ear basement membrane at the end of fresh specimens, using atomic force microscopy in the liquid contact measurement, to obtain the force curve of different parts. After calculation, corresponding to the Corti device is equivalent to Hensen cells, outer hair cells, columnar cells, inner hair cells, inner cells, parts of the cover membrane and the basement membrane of the local, the Young’s Modulus were 46 ± 1.7,59 ± 0.9, 250 ± 31, 140 ± 2.8, 430 ± 29.9, 210 ± 7.2 and 230 ± 8.8 kPa. The results show that there are significant differences in the Young’s modulus of the basement membrane in the radial arrangement. Measuring the Young’s modulus of each structure in the whole basement membrane specimen can more accurately reflect their elasticity under physiological conditions feature.