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目的:研究红花石蒜中的石蒜生物碱。方法:采用多种层析柱分离手段,运用NMR和HR-ESI-MS等波谱技术鉴定化合物的结构。此外,生物碱110进行了流感甲型病毒的活性测试。结果:从红花石蒜中分离鉴定了1个新石蒜生物碱和9个已知的石蒜生物碱:2-methoxy-6-O-ethyloduline(1),2-methoxy-6-O-methyloduline(2),trispherine(3),8-O-demethylho-molycorine(4),homolycorine(5),9-O-demethylhomolycorine(6),oduline(7),lycorenine(8),6α-O-methyllycorenine(9)和O-ethyllycorenine(10)。结论:化合物1为新的高石蒜碱类型的石蒜生物碱,生物碱13为该植物中的主要成分,且对流感甲型病毒显示了较弱的抗病毒活性,IC50分别为2.06,0.69,2.71μgmL1,CC50分别为14.37,4.79,80.12μgmL1。
Objective: To study Lycoris alkaloids in Lycoris radix. Methods: A variety of chromatographic column separation methods were used to identify the structures of the compounds by NMR and HR-ESI-MS. In addition, alkaloids 110 were tested for the activity of influenza A virus. Results: One new Lycoris radiate Alkaloids and nine known Lycoris alkaloids were isolated and identified from the Lycoris radiata: 2-methoxy-6-O-ethyloduline (1) methyloduline (2), trispherine (3), 8-O-demethylho-molycorine (4), homolycorine (5), 9-O-demethylhomolycorine (6), oduline (7), lycorenine (9) and O-ethyllycorenine (10). CONCLUSION: Compound 1 is a new Lycoris alkaloid of Lycorine. Alkaloid 13 is the main component of this plant and shows weak antiviral activity against Influenza A virus with IC50 of 2.06, 0.69, 2.71μgmL1, CC50 were 14.37,4.79,80.12μgmL1.