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分析血红素加氧酶(heme oxygenase,HO)基因mRNA加帽位点上游1416bp及mRNA5′端未翻译区24bp全长1.44kb片段。通过大片段缺失,发现与诱导剂相关的mRNA加帽位点近端上游121bp,可使基因的瞬时表达提高3～5倍,同时诱导作用发生在SV_(40)增强子元件位于启动子序列上游。另外在1.44kb片段间mRNA加帽位点上游有沉默子或负调控元件及NF-κB-和AP-2结合位点,而在1.44kb之外还有附加的诱导增强子元件。
The full-length 1.44 kb fragment of 1416bp upstream of the capsid site of heme oxygenase (HO) gene and the untranslated region of mRNA 5 ’end was analyzed. Through the large fragment deletion, it was found that the transient expression of gene was up to 3 ~ 5 times higher than the proximal 121 bp upstream of the decoy-related mRNA capsid site, and the SV_ (40) enhancer element was located upstream of the promoter sequence . In addition, silencer or negative regulatory elements and NF-κB- and AP-2 binding sites were located upstream of the 1.44 kb inter-mRNA capping site, with additional inducible enhancer elements beyond 1.44 kb.