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利用根癌农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｔｕｍｅｆａｃｉｅｎｓ）介导法将豇豆胰蛋白酶抑制剂（Ｃｏｗ－ｐｅａＴｒｙｐｓｉｎＩｎｈｉｂｉｔｏｒ，ＣｐＴＩ）基因转移进入棉花（ＧｏｓｙｐｉｕｍｈｉｒｓｕｔｕｍＬ．）。棉花幼苗的下胚轴与携带有ＣｐＴＩ基因和Ｎｐｔ－Ⅱ基因的根癌农杆菌共培养，在选择培养基上诱导出了卡那霉素抗性（Ｋｍｒ）愈伤组织。选择Ｎｐｔ－Ⅱ阳性的胚性愈伤组织在胚状体诱导培养基上进行胚状体诱导，继而在分化培养基上进行植株分化，最终获得了再生棉花植株。经Ｎｐｔ－Ⅱ分析、ＰＣＲ及Ｓｏｕｔｈｅｒｎ检测证明，外源ＣｐＴＩ基因和标记基因（Ｎｐｔ－Ⅱ基因）存在于转化棉株及其后代中。抗棉铃虫（Ｈｅｌｉｏｔｈｉｓａｒｍｉｇｅｒａ）生物活性检测表明转基因植株后代具有明显的抗棉铃虫能力。
The cowpea trypsin inhibitor (CpTI) gene was transferred into cotton (Gosypium hirsutum L.) using Agrobacterium tumefaciens mediated method. The hypocotyls of cotton seedlings were cocultivated with Agrobacterium tumefaciens carrying the CpTI gene and the Npt-II gene, and kanamycin resistant (Kmr) callus was induced on selection medium. Npt-Ⅱ-positive embryogenic callus was selected for embryoid body induction on embryoid body induction, followed by differentiation on the differentiation medium, and finally regenerated cotton plants. Npt-Ⅱ analysis, PCR and Southern blotting showed that exogenous CpTI gene and marker gene (Npt-Ⅱgene) existed in the transformed cotton plants and their offspring. Helicobacter pylori (Heliothis armigera) bioassay showed that the progenies of transgenic plants had obvious anti-bollworm ability.