论文部分内容阅读
目的研究重组小鼠IL-13对肺成纤维细胞的作用及其机制。方法取生长良好的NIH/3T3,给予无血清培养基饥饿24 h,在培养液中加入IL-13。MTT法、RT-PCR等方法测定肺成纤维细胞的增殖及TGF-β1、PDGF-AA、-αSMA等因子的表达。MTT法观察TGF-β单克隆抗体干预后,IL-13对NIH/3T3细胞增殖反应的影响。结果与对照组相比,IL-13促进NIH/3T3细胞增殖(P<0.05)。IL-13能够显著地促进NIH/3T3细胞中TGF-β1和PDGF-AA mRNA的表达,刺激NIH/3T3分泌-αSMA。TGF-β单克隆抗体能抑制IL-13诱导引起的NIH/3T3细胞增值。结论IL-13能促进成纤维细胞的增殖,其可能的机制是通过促进成纤维细胞分泌TGF-β1和PDGF-AA来发挥作用。
Objective To study the effect of recombinant mouse IL-13 on lung fibroblasts and its mechanism. Methods The well-growing NIH / 3T3 cells were starved for 24 h in serum-free medium and IL-13 was added to the medium. MTT and RT-PCR were used to determine the proliferation of lung fibroblasts and the expression of TGF-β1, PDGF-AA and -αSMA. The effect of IL-13 on the proliferation of NIH / 3T3 cells was observed by MTT assay after the intervention of TGF-β monoclonal antibody. Results Compared with the control group, IL-13 promoted the proliferation of NIH / 3T3 cells (P <0.05). IL-13 significantly promoted the expression of TGF-β1 and PDGF-AA mRNA in NIH / 3T3 cells, stimulating NIH / 3T3 secretion-αSMA. The TGF-β monoclonal antibody inhibits NIH / 3T3 cell proliferation induced by IL-13. Conclusion IL-13 can promote the proliferation of fibroblasts. The possible mechanism is that IL-13 can promote the secretion of TGF-β1 and PDGF-AA by fibroblasts.