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Mi-1.2基因编码CC-NBS-LRR家族抗病蛋白,对线虫及刺吸式昆虫具有广谱抗性。以番茄木虱和携带Mi-1.2基因的番茄品系‘Motelle’作为研究体系,研究番茄木虱取食对番茄叶片抗氧化酶活性及防御反应相关基因表达的影响。结果表明:番茄木虱的取食显著提高了番茄叶片抗氧化酶POD、CAT、APX等的活性,同时诱导Mi-1.2基因表达;番茄木虱的取食上调SA合成有关基因PAL、SA信号途径标志基因PR-1(P4)及与Mi-1.2功能相关基因WRKY70、WRKY72a、WRKY72b的表达。JA/systemin信号途径和伤害有关的Pin II、TPI-1、PR-6、PR-7、PR-5等及Prosystemin编码基因Psy的表达未发生变化;JA合成途径相关酶编码基因AOS2、Lox D下调表达,AOC的表达没有变化。SA信号途径可能参与Mi-1.2介导的番茄对于刺吸式昆虫番茄木虱的防御反应。另外,Mi-1.2的RNAi沉默不会改变上述基因的m RNA水平,说明Mi-1.2基因产物对这些基因的表达无调控作用。
The Mi-1.2 gene encodes the CC-NBS-LRR family of disease-resistant proteins with broad-spectrum resistance to nematodes and sucking insects. To study the effect of tomato psyllid lodging and the tomato line Mi-1.2 ’Motelle’ on the antioxidant enzyme activity and defense-related gene expression in tomato leaves. The results showed that the feeding of tomato psyllid significantly increased the activity of POD, CAT, APX and other enzymes in tomato leaves and induced the expression of Mi-1.2 gene. The feeding of tomato psylla increased the synthesis of SA and PAL, SA signal pathway PR-1 (P4) and the expression of WRKY70, WRKY72a and WRKY72b related to Mi-1.2. The expressions of Pin II, TPI-1, PR-6, PR-7, PR-5, and Psy of Prosystemin did not change in the pathways of JA / systemin signaling pathway. The AOS2, Lox D Down-regulated expression, AOC expression did not change. SA signaling pathway may be involved in the defense response of Mi-1.2-mediated tomato to the sucking insects tomato psylla. In addition, Mi-1.2 RNAi silencing does not change the m RNA level of the above genes, indicating that Mi-1.2 gene product has no regulation on the expression of these genes.