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采用高效液相色谱法,以丁烯基苯酞为内参物,建立川芎中1种酚酸类成分和5种苯酞类成分的单标多组分HPLC定量分析方法,并考察和验证该方法在川芎质量评价中应用的可行性和准确性。实验所用流动相为乙腈-0.2%甲酸水溶液,流速为1.0 m L·min-1,柱温30℃,检测波长分别为252 nm(阿魏酸、藁本内酯、丁烯基苯酞)和266 nm(洋川芎内酯I、洋川芎内酯A、阿魏酸松柏酯),进样量20μL,梯度洗脱。结果显示,单标多组分HPLC定量分析法计算所得值与外标法实测值之间无显著性差异,RSD值均小于5%;相对校正因子的耐用性较好,色谱条件改变后,相对校正因子变化的RSD值均小于5%。所建立的单标多组分HPLC定量分析法可准确地用于中药川芎的多指标同步质量控制。
A high-performance liquid chromatography (HPLC) method with butenylphthalide as an internal reference substance was established for the determination of one phenolic acid component and five phthalide components in Chuanxiong by single-label, multi-component HPLC quantitative analysis method. The feasibility and accuracy of the application in the quality evaluation of. The mobile phase used in the experiment was acetonitrile-0.2% formic acid aqueous solution, the flow rate was 1.0 m L · min-1, the column temperature was 30 ℃ and the detection wavelength was 252 nm (ferulic acid, ligustilide, butenylphthalide) 266 nm (Yang Chuanxiong lactone I, Yang Chuanxiong lactone A, ferulic acid coniferyl ester), injection volume 20 μL, gradient elution. The results showed that there was no significant difference between the single-standard and multi-component HPLC quantitative analysis method and the external standard method, the RSD values were less than 5%. The relative calibration factor was more durable. When the chromatographic conditions were changed, Correction factor changes in RSD values were less than 5%. The established single-standard multi-component HPLC quantitative analysis method can be accurately used for multi-index synchronized quality control of Chuanxiong.