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目的：评价抗凋亡基因ｂｃｌ－２（βｃｅｌ１ｙｍｐｈｏｍａ／ｌｅｕｋｅｍｉａ－２）蛋白过量表达在葡萄膜与结膜黑色素瘤发生发展过程中的意义。方法：应用流式细胞术（ｆｌｏｗｃｙｔｏｍｅｔｒｙ，ＦＣＭ）及细胞免疫荧光染色技术对４０例葡萄膜恶性黑色素瘤（ｕｖｅａｌｍａｌｉｇ－ｎａｎｔｍｅｌａｎｏｍａ，ＵＭＭ）、５例结膜色素痣（ｃｏｎｊｕｎｃｔｉｖａｌｎｅｖｕｓ，ＣＮ）及７例结膜恶性黑色素瘤（ｃｏｎｊｕｎｃｔｉｖａｌｍａｌｉｇｎａｎｔｍｅｌａｎｏｍａ，ＣＭＭ）的ｂｃｌ－２蛋白表达进行定量检测。结果：ＣＭＭ较ＣＮ的ｂｃｌ－２蛋白表达量明显增加（Ｐ＜０．０５）；ＣＭＭ及ＵＭＭ的ｂｃｌ－２蛋白表达阳性率分别为８５．７１％及７２．５０％；ＵＭＭ的ｂｃｌ－２蛋白表达与病理类型、巩膜受侵、睫状体受累、肿瘤大小均不相关（Ｐ＞０．０５）。结论：ｂｃｌ－２蛋白可能通过抑制细胞凋亡（ａｐｏｐｔｏｓｉｓ）过程而参加ＣＭＭ与ＵＭＭ的发生；ｂｃｌ－２表达量可能有助于ＣＮ与ＣＭＭ的鉴别诊断，但在ＵＭＭ病理恶性程度评估上的价值不大。
Objective: To evaluate the significance of over-expression of anti-apoptotic gene bcl-2 (βcel1ymphoma / leukemia-2) in the development of uveal and conjunctival melanoma. Methods: 40 cases of uvealmalig-nantmelanoma (UMM), 5 cases of conjunctival nevus (CN) and 7 cases of conjunctival malignant melanin were treated with flowcytometry (FCM) and immunofluorescence staining Bcl-2 protein expression in the tumor (conjunctivalmalignantmelanoma, CMM) was quantified. Results: The expression of bcl-2 in CMM was significantly higher than that in CN (P <0.05). The positive rates of bcl-2 protein in CMM and UMM were 85.71% and 72.50% Protein expression and pathological type, scleral invasion, ciliary body involvement, tumor size were not related (P> 0.05). CONCLUSION: The bcl-2 protein may participate in the pathogenesis of CMM and UMM by inhibiting the process of apoptosis. The expression of bcl-2 may contribute to the differential diagnosis between CN and CMM, but the value of evaluating the malignant degree of UMM Not big.