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目的：进一步分离在鼻咽癌组织中表达下调／缺失的候选抑瘤基因。方法：采用PCR方法对有差异表达的cDNA序列（AF152605和AF091517）进行探针标记，Northern杂交确定其mRNA转录本大小，进而混合两种PCR探针对骨骼肌cDNA文库进行筛选，阳性克隆经PCR鉴定后直接测序。结果：克隆了转录本为2．2Kb、1．1Kb和1．4Kb三个基因，GenBank登录号分别为AF179285、AF170307和AF194971。结论：混合探针文库筛选法是同时、快速获得多个cDNA片段其全长序列的可借鉴实验手段。
OBJECTIVE: To further isolate candidate tumor suppressor genes with down-regulation/deletion in nasopharyngeal carcinoma tissues. Methods: Differentially expressed cDNA sequences (AF152605 and AF091517) were probed with PCR and the mRNA transcript size was determined by Northern hybridization. Two PCR probes were then used to screen the skeletal muscle cDNA library. Positive clones were obtained by PCR. Identification after direct sequencing. RESULTS: The transcripts were 2.2Kb, 1.1Kb and 1.4Kb. The GenBank accession numbers were AF179285, AF170307 and AF194971, respectively. Conclusion: The mixed probe library screening method can be used as a reference for the simultaneous and rapid acquisition of multiple cDNA fragments.