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目的和方法：采用生物素标记阿黑皮素原（POMC）cRNA探针（Bio－cRNA）原位来交法结合图象分析，从基因转录水平现农长时期（共7周）大强度（速度由15m／min递增至35m／min，运动时间为20-25min/d）跑台运动的大鼠不同防区PONCmRNA表达。结果：该强度下大鼠末次运动结束后POMC基因表达在额叶皮层、海马CA1下降（前者至运动结束后3h仍未恢复，后者至运动结束后对30min即恢复），在下丘脑则增强。结论：POMCmRNA表达在该强度下运动的大鼠不同脑区呈现不同的动态变化趋势。
Objective and Methods: Using biotin-labeled POMC cRNA probe (Bio-cRNA) in situ hybridization with image analysis, from the gene transcription level is now agricultural long period (a total of 7 weeks) high intensity ( Speed increased from 15m / min to 35m / min, exercise time is 20-25min / d) treadmill exercise in different zones of PONCmRNA expression. Results: At the end of the last exercise, the POMC gene expression decreased in the cortex and hippocampus at the end of this exercise (the former did not recover after 3 hours and the latter recovered 30 minutes after the end of exercise), but increased in the hypothalamus. CONCLUSION: Different brain regions of POMCmRNA expression rats exercising at this intensity show different trends.