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目的 探讨γ干扰素抑制血管内膜增生和防治血管再狭窄的体内机制。方法 建立兔血管狭窄模型 ,动态观察肌内给予重组γ干扰素对损伤后不同时期血管内膜平滑肌细胞 (vSMCs)原位表达血小板源性生长因子B链mRNA(PDGF BmRNA)和增殖细胞核抗原的影响。结果 γ干扰素显著抑制 1周和 4周时内膜vSMCs表达增殖细胞核抗原 ,抑制率分别为 88 5 %和 5 8 9% ;显著抑制各观察时期新生内膜表达PDGF BmRNA。结论 γ干扰素通过下调vSMCs原位表达PDGF BmRNA抑制血管vSMCs的增殖和内膜增生 ,可能用于临床再狭窄的防治。
Objective To investigate the in vivo mechanism of interferon-gamma (IFN-γ) in inhibiting intimal hyperplasia and preventing vascular restenosis. Methods Rabbit vascular stenosis model was established to observe the effect of intramuscular administration of recombinant interferon gamma on in situ expression of platelet-derived growth factor B chain mRNA (PDGF B mRNA) and proliferating cell nuclear antigen (PCNA) in vascular smooth muscle cells (vSMCs) . Results Gamma interferon significantly inhibited the expression of proliferating cell nuclear antigen in endometrial vSMCs at 1 week and 4 weeks, with inhibitory rates of 88.5% and 58.9%, respectively. It significantly inhibited the expression of PDGF B mRNA in neointima during all observation periods. Conclusion IFN-γ inhibits vSMCs proliferation and intimal hyperplasia by downregulation of PDGF BmRNA in vSMCs, which may be used in the prevention and treatment of clinical restenosis.