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目的评价广西不同产地钩藤Uncaria rhynchophylla的药材质量,确定药材的最佳采收期。方法采用HPLC测定28批不同产地和1个产地不同时期钩藤药材钩藤碱的含量,采用Gemini C18色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(含0.002 mol.L-1三乙胺,用冰乙酸调pH7.5)(64∶36),流速1.0 mL.min-1,检测波长254 nm。结果 28批不同产地钩藤药材中有11批药材能检出钩藤碱,但含量差异较大;有17批药材未能检出钩藤碱成分;不同时期钩藤中钩藤碱的动态积累差异较大。结论广西不同产地钩藤药材的质量有一定差异;钩藤药材于秋、冬二季采收似乎较合理。
Objective To evaluate the medicinal quality of Uncaria rhynchophylla from different areas in Guangxi and determine the best harvest time of medicinal materials. Methods HPLC was used to determine the content of rhynchophylline in 28 batches of Rhizoma et Radix notoginseng at different time points. Gemini C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was methanol-water (containing 0.002 mol·L -1 triethylamine, adjusted to pH7.5 with glacial acetic acid (64:36), the flow rate was 1.0 mL.min-1 and the detection wavelength was 254 nm. RESULTS: Rhynchophylline was detected in 11 batches of Rhynchophyllotaceae from 28 batches of different origins, but its content was quite different. There were 17 batches of herbs failed to detect the rhynchophylline components. The dynamic accumulation of rhynchophylline big different. Conclusion There are some differences in the quality of Uncaria rhynchophylla from different areas in Guangxi. The harvest of Uncaria macrophylla seems to be reasonable in autumn and winter.