论文部分内容阅读
目的探讨组织芯片技术在免疫组化试验中的可靠性和提高组织芯片可信性的方法。方法选择82例人乳腺癌标本石蜡蜡块,利用传统制片技术和自行研制的专用器具分别制作常规石蜡切片和组织芯片,采用免疫组化技术检测雌激素受体(ER)、孕激素受体(PR)的表达。结果采用自行研制的专用器具制备组织芯片,所得样本的可分析率均在92.9%以上,且随着对同一标本取材数量的增加,其可分析标本率也明显上升。采用传统方法检测的82例乳腺癌标本中ER和PR的阳性率分别为61%和58.5%;采用组织芯片技术,一式一份取材,其阳性率分别为51.9%和50.0%,一式两份方式取材,其阳性率分别为53.8%和53.8%,采用一式三份方式取材,其阳性率分别为57.1%和60.7%,且三种不同取材方式的检测结果与传统制片法之间并无统计学差异(P>0.75)。结论在采用统一制备标准并保证组织芯片质量的前提下,挖取直径1.5mm的组织样本制备组织芯片,尽管组织芯片技术与传统方法检测的结果的一致率仍随着对同一标本取材次数的增加而提高,但是三种不同取材方式的检测结果之间并无统计学差异。因此,一式一份取材制备组织芯片完全可替代传统制片技术用于免疫组织化学乃至原位杂交、荧光原位杂交技术的研究,且可保证组织芯片的可信性和高通量特征。
Objective To investigate the reliability of tissue microarray in immunohistochemistry and the method to improve the reliability of tissue microarray. Methods Eighty paraffin wax blocks were selected from human breast cancer specimens. The conventional paraffin sections and tissue microarrays were prepared by using traditional techniques and special instruments developed by ourselves. Immunohistochemistry was used to detect the expressions of estrogen receptor (ER), progesterone receptor (PR) expression. Results Tissue microarrays were prepared by using the special equipment developed by ourselves. The analyzable rates of all the samples obtained were over 92.9%. And with the increase of the number of samples taken from the same sample, the rate of analyzable samples also increased obviously. The positive rates of ER and PR in 82 cases of breast cancer detected by traditional methods were 61% and 58.5% respectively. The positive rates of ER and PR were 51.9% and 50.0% The positive rates were 53.8% and 53.8% respectively. The positive rates were 57.1% and 60.7% respectively. There was no statistical difference between the results of three different methods and traditional methods Learning difference (P> 0.75). Conclusion Tissue samples with a diameter of 1.5 mm were obtained by using uniform preparation standards and ensuring the quality of the tissue chips. Although the coincidence rate between the tissue chip technology and the traditional method was still increasing with the number of samples taken from the same sample But increased, but there is no statistical difference between the three different methods of detection. Therefore, a single preparation of tissue chips can completely replace the traditional production technology for immunohistochemistry and in situ hybridization, fluorescence in situ hybridization technology, and to ensure the credibility of the tissue chip and high-throughput characteristics.