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基因型Ｈｂ，Ｍａ和Ｍｃ的火炬松成熟合子胚在附加１．０ｍｇ／ＬＮＡＡ，４．０ｍｇ／ＬＢＡ，５００ｍｇ／ＬＬＨ和５００ｍｇ／Ｌ谷氨酰胺的ＴＥ培养基上培养１２周后，在子叶和胚轴部位形成不定芽原基。然后将合子胚转移到附加０．５ｍｇ／ＬＮＡＡ，０．５ｍｇ／ＬＩＢＡ，２ｍｇ／ＬＢＡ，５００ｍｇ／ＬＬＨ和５００ｍｇ／Ｌ谷氨酰胺的ＴＥ不定芽分化培养基上，６周后分化产生大量不定芽。３种基因型中，Ｈｂ的直接不定芽分化频率最高，达５８．２％。直接不定芽经伸长培养６周后，转移到附加０．１ｍｇ／ＬＩＢＡ，１．０ｍｇ／ＬＢＡ和０．５ｍｇ／ＬＧＡ３的ＴＥ生根培养基上，６周后直接不定芽的最高生根频率达４６％。
Pinus taeda mature embryonic embryos of genotypes Hb, Ma and Mc were cultured on TE medium supplemented with 1.0 mg / L NAA, 4.0 mg / L BA, 500 mg / L HH and 500 mg / L glutamine for 12 weeks, Hypocotyl sites form adventitious buds primordia. Zygotic embryos were then transferred to TE adventitious bud differentiation medium supplemented with 0.5 mg / L NAA, 0.5 mg / L IBA, 2 mg / L BA, 500 mg / L HH and 500 mg / L glutamine, with a large number of adventitious buds differentiated after 6 weeks . Among the three genotypes, Hb had the highest frequency of adventitious buds differentiation, reaching 58.2%. Direct adventitious buds were cultured for 6 weeks and transferred to TE rooting medium supplemented with 0.1 mg / L IBA, 1.0 mg / L BA and 0.5 mg / L GA3. After 6 weeks, the highest rooting frequency of adventitious buds reached 46 %.