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研究用Ｐ２Ｘ_１，Ｐ２Ｘ_２，Ｐ２Ｘ_３，Ｐ２Ｘ_４，Ｐ２Ｘ_５和Ｐ２Ｘ_６受体的多克隆抗体，ＰＡＰ免疫组织化学及ＤＡＢ终产物金银加强法检查了大鼠味蕾和邻近组织中 Ｐ2Ｘ受体的分布以探究 ＡＴＰ在味觉激信号传导中的可能作用。结果：在轮廓乳头、菌状乳头和叶扶乳头的味蕾内显示出许多Ｐ２Ｘ_３免疫反应阳性神经纤维，多里含珠状，有的伸到味孔处。在无味蕾的粘膜上皮中，也发现少量Ｐ２Ｘ_２免疫反应阳性神经纤维。在味蕾下的粘膜固有层中，近上皮基膜Ｐ２Ｘ_３免疫反应用性神经纤维束，在乳头中固有层中有Ｐ２Ｘ_３免疫反应阳性神经纤维网，在乳头周围的肌组织中也显示出Ｐ２Ｘ_３免疫反应阳性神经纤维束。 Ｐ２Ｘ_３受体免疫活性的分布特征与 Ｐ２Ｘ_３的一致，但着色比 Ｐ２Ｘ_３的浅得多。结果表明ＡＴＰ可能是味蕾味觉细胞中的神经递质，ＡＴＰ通过激活轴突上Ｐ２Ｘ受体将味觉信号传导给味觉传入神经。
We investigated the distribution of P2X receptors in the taste buds and adjacent tissues of rats using polyclonal antibodies to P2X_1, P2X_2, P2X_3, P2X_4, P2X_5 and P2X_6 receptors, PAP immunohistochemistry, and gold-silver strengthening of the DAB end products to explore the role of ATP in The possible role of taste stimulus transmission. RESULTS: There were many P2X 3 immunoreactive positive nerve fibers in the taste buds of contour nipples, fungal papillae and papillae. In the tasteless bud mucosal epithelium, a small amount of P2X_2 immunoreactive nerve fibers were also found. In the mucosal lamina propria under the taste buds, P2X_3 immunoreactive nerve fiber bundles near the epithelial basement membrane, P2X_3 immunoreactive positive nerve fiber network in the lamina propria of the nipple, and P2X_3 immunoreactivity in the muscle tissue around the nipple Positive nerve fiber bundle. The distribution of P2X_3 receptor immunoreactivity was consistent with that of P2X_3, but the staining was much lighter than that of P2X_3. The results suggest that ATP may be a neurotransmitter in the taste buds of taste buds. ATP activates taste signals to the sensory afferent nerves by activating P2X receptors on axons.