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实验用成熟母羊在动情期(14,16天)时杀死,另一组母羊交配后于胚胎种植14天及妊娠23天时杀死.实验动物分成三组:对照组未给药,杀死后立即摘出内膜及孕体储存于-20℃,另一部分孵育于培养基中.第二组:从母羊交配后第七天开始,每日皮下注射消炎痛,每日三次,每次100mg,第三组静脉注射乙酰水杨酸,每日二次每次500mg,直至第二十二天杀死取出内膜及胚胎.用放射免疫法测定各组织中PG含量.结果:在动情期及妊娠期14天时子宫内膜PGF_(2α)及PGE_2的浓度相近似(动情期PGF_(2α)及PGE_2各为263±42及235±46;妊娠早期各为390±43及318±60pg/mg).在黄体裂解期(动情期16天)时
The mature ewes were killed in estrus (14 and 16 days), and the other ewes were killed after embryo implantation on day 14 and 23 days of gestation.The experimental animals were divided into three groups: the control group was not given, killed Immediately after death, the endometrium and the pregnant body were removed and stored at -20 ° C, and the other part was incubated in the medium.Second group: From the seventh day after the mating of the ewes, daily indomethacin was injected subcutaneously three times a day 100mg, the third group of intravenous injection of acetylsalicylic acid twice a day for each 500mg, until the day of death to remove the endometrial and embryo.Radioimmunoassay was used to determine the content of PG in each tissue.Results: During estrus And PGF 2α and PGE 2 concentrations in endometrium at 14 days of gestation (263 ± 42 and 235 ± 46 in puberty and 390 ± 43 and 318 ± 60 pg / mg in early gestation respectively) ) During luteal lysis (estrus 16 days)