论文部分内容阅读
Self-assembly is a powerful means to create new materials and new catalysts.The advantages of biological self-assembly are based on it being highly programmable and prone to multilevel reg-ulation,which can lead to multiple and complex functions.The self-assembly of carboxysomes in cyanobacteria enables the carboxysomes to enrich carbon dioxide in their interior,resulting in the formation of a highly efficient,multiple-enzyme catalytic system.Here,we show that the construction and coexpression of all genes of the β-carboxysome from the cyanobacterium Thermosynechococcus elongatus BP-1 can lead to the production of β-carboxysome-like structures in Escherichia coli.These shell structures were characterized intracellularly and extracellularly by transmission electron microscopy.This work lays a foundation for understanding carboxysome assembly and catalysis and the development of novel carboxysome-based nanomaterials utilizing synthetic biology.