hsa_circ_0062019 promotes the proliferation,migration,and invasion of prostate cancer cells via the

• 作 者: Peiyu Wang Ligang Zhang Shuiping Yin Yuchen Xu Sheng Tai Li Zhang Chaozhao Liang
• 机 构: Department of Urology,The First Affiliated Hospital of Anhui Medical University,Hefei 230022,China;I
• 出 处: 生物化学与生物物理学报（英文版）
• 发表日期: 2021年7期
• 关键词: prostate cancer hsa_circ_0062019 competing endogenous RNA miR-195-5p high mobili

Circular RNA (circRNA) is a new class of non-coding RNA.It was reported that circRNA involves in the metastasis of cancer.The aim of this study is to explore the role and mech-anism of circRNA hsa_circ_0062019 in the development of prostate cancer (PCa).Our results showed that hsa_circ_0062019 was highly expressed in PCa cell lines.Cell Counting Kit-8 assay revealed that upregulation of hsa_circ_0062019 boosted PCa cell proliferation,and silencing of hsa_circ_0062019 inhibited cell proliferation.Meanwhile,transwell assay proved that upregula-tion of hsa_circ_0062019 facilitated PCa cell invasion and migration,while downregulation of hsa_circ_0062019 inhibited these malignant phenotypes.Furthermore,luciferase reporter assay proved the binding of hsa_circ_0062019 with miR-195-5p and the binding between miR-195-5p and high mobility group AT-hook 2 (HMGA2),suggesting that hsa_circ_0062019 promoted the expression of HMGA2 by sponging miR-195-5p.In addition,our results revealed that the hsa_circ_0062019-induced PCa cell malignant phenotypes were notably reversed by the down-regulation of HMGA2.Overall,our study demonstrated that hsa_circ_0062019 promoted PCa cell proliferation,migration,and invasion via upregulation of HMGA2 expression by sponging miR-195-5p.Our study proved a novel molecular mechanism of PCa development and provided a potential target for the treatment of PCa.