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以橡胶树未成苗体细胞胚为试材,选取未成苗双子叶胚、连体胚、多子叶胚、单子叶胚和子叶愈伤化胚作为外植体,研究不同体细胞胚类型的外植体分化茎芽的情况,并利用其进行幼态微型芽条的培育研究。结果表明:在植株诱导培养基中培养80d后,未成苗双子叶胚、连体胚、多子叶胚、单子叶胚、子叶愈伤化胚均能分化出芽,双子叶胚分化率最高(达90%),单子叶胚最低(20%),多子叶胚和连体胚最多可分化出芽3个,双子叶胚2个。幼态微型芽条增殖的最优培养基为MS+6-BA2mg·L-1或MS+6-BA2mg·L-1+KT1mg·L-1,而添加NAA对芽的伸长有抑制作用;适宜幼态微型芽条伸长培养的基本培养基为MS。
Using the somatic embryos of immature seedlings of rubber tree as tested material, the explants of different somatic embryo types were selected as the explants of unformed seedlings of dicotyledonous, conjoined, multi-cotyledon, monocotyledon and cotyledon callus Differentiation of stem buds, and the use of its juvenile micro-sprout cultivation studies. The results showed that all the embryogenic calluses of dicotyledon, conjoined, multi-cotyledon, monocotyledon and cotyledon were able to differentiate into buds after culture for 80 days in plant-induced medium, with the highest rate of dicotyledonous embryo %), The lowest single leaf embryo (20%), multi-cotyledons and Siamese embryos can differentiate up to three buds, two dicotyledons embryo. The optimal culture medium for juvenile micro-sprout proliferation was MS + 6-BA2mg · L-1 or MS + 6-BA2mg · L-1 + KT1mg · L-1, while adding NAA could inhibit the elongation of shoots. Suitable for young juvenile bud elongation elongation of the basic medium for the MS.